Anti-MDC1抗体(ab114143)

概述

  • 产品名称Anti-MDC1抗体
    参阅全部 MDC1 一抗
  • 描述
    兔多克隆抗体to MDC1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Cow, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1950 - 2050 of Human MDC1.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HEK293; MCF7; Caco2.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab114143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 238 kDa (predicted molecular weight: 227 kDa).
ICC/IF 1/200.

靶标

  • 功能Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
  • 组织特异性Highly expressed in testis.
  • 序列相似性Contains 2 BRCT domains.
    Contains 1 FHA domain.
  • 结构域Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.
  • 翻译后修饰Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.
  • 细胞定位Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Homologue to Drosophila photoreceptor protein calphotin antibody
    • MDC 1 antibody
    • Mdc1 antibody
    • MDC1_HUMAN antibody
    • Mediation of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint protein 1 antibody
    • NFBD 1 antibody
    • NFBD1 antibody
    • Nuclear factor with BRCT domains 1 antibody
    • Nuclear Factor with BRCT Domains Protein 1 antibody
    see all

Anti-MDC1 antibody 图像

  • All lanes : Anti-MDC1 antibody (ab114143) at 1 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 227 kDa
    Observed band size : 238 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 43 kDa,57 kDa,72 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ab114143 (1/200) staining MDC1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5%Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

Anti-MDC1 antibody (ab114143)参考文献

ab114143 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 Oct 22 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"