概述

  • 产品名称Anti-MDC1抗体
    参阅全部 MDC1 一抗
  • 描述
    兔多克隆抗体to MDC1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide, which represents a portion of the human Mediator of DNA Damage Checkpoint Protein 1 encoded within exon 5 (LocusLink ID 9656).

  • 阳性对照
    • Tested with HeLa cells and HEK 293 cells.
  • 常规说明

    In response to recent feedback about mouse species we no longer guarantee this anymore. Please do contact our scientific support team for more information.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antibodies were affinity purified using the peptide immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab11169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
WB 1/500 - 1/2500. Detects a band of approximately 250 kDa (predicted molecular weight: 220 kDa).

靶标

  • 功能Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
  • 组织特异性Highly expressed in testis.
  • 序列相似性Contains 2 BRCT domains.
    Contains 1 FHA domain.
  • 结构域Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.
  • 翻译后修饰Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.
  • 细胞定位Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Homologue to Drosophila photoreceptor protein calphotin antibody
    • MDC 1 antibody
    • Mdc1 antibody
    • MDC1_HUMAN antibody
    • Mediation of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint protein 1 antibody
    • NFBD 1 antibody
    • NFBD1 antibody
    • Nuclear factor with BRCT domains 1 antibody
    • Nuclear Factor with BRCT Domains Protein 1 antibody
    see all

Anti-MDC1 antibody 图像

  • ab11169 staining MDC1 in HCT116 cells treated with GANT 61 (ab120904), by ICC/IF. Increase in MDC1 expression correlates with increased concentration of GANT 61, as described in literature.
    The cells were incubated at 37°C for 4 hours in media containing different concentrations of ab120904 (GANT 61) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11169 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.


  • Predicted band size : 220 kDa

    Samples: A) Nuclear extract (50 mcg) from HeLa cells. B) Whole cell lysate from HEK 293 cells. Antibodies: A) ab11169 used at the indicated concentrations for WB. B) ab11170 (column labelled BL579) and ab11171 (column labelled BL580) (a ) used at 3.3 mcg/mg lysate for IP followed by WB using ab11169 at 0.1 mcg/ml. Detection: Chemiluminescence with exposure times less than 5 min.

    Samples: A) Nuclear extract (50 mcg) from HeLa cells. B) Whole cell lysate from HEK 293 cells. Antibodies: A) ab11169 used at the indicated concentrations for WB. B) ab11170 (column labelled BL579) and ab11171 (column labelled BL580) (a ) used at 3.3 mcg/mg lysate for IP followed by WB using ab11169 at 0.1 mcg/ml. Detection: Chemiluminescence with exposure times less than 5 min.

  • ab11169 staining MDC1 in human cervical cancer cells (HeLa) by ICC/IF (immunocytochemistry/immunofluorescence - left image). Cells were paraformaldehyde fixed, permeabilized with Triton X-100 and blocked with 5% BSA for 1 hour at 25°C. The primary antibody (1/200) was incubated with the sample for 12 hours at 4°C. An Alexa Fluor 594®-conjugated Chicken anti-rabbit polyclonal (1/2000) was used as the secondary. The nuclei were stained with DAPI (right image).

    See Abreview

  • Immunofluorescence analysis of A549 cells before (left) and after ionizing radiation (right), staining MDC1 with ab11169.

    Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% BSA for 30 minutes at room temperature. Cells were incubated with primary antibody for 1 hour at room temperature. A Cy2®-conjugated anti-rabbit IgG was used as the secondary antibody.

Anti-MDC1 antibody (ab11169)参考文献

This product has been referenced in:
  • Leung JW  et al. Nucleosome acidic patch promotes RNF168- and RING1B/BMI1-dependent H2AX and H2A ubiquitination and DNA damage signaling. PLoS Genet 10:e1004178 (2014). Human . Read more (PubMed: 24603765) »
  • Liang H  et al. Homeostatic control of polo-like kinase-1 engenders non-genetic heterogeneity in G2 checkpoint fidelity and timing. Nat Commun 5:4048 (2014). WB ; Human . Read more (PubMed: 24893992) »

See all 16 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Treatment IR
Specification HEK293
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 26 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - 0.2% Triton X-100
Specification HeLa
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 06 2016

Abreviews
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (7.5%)
Sample Mouse Cell lysate - other (MEF)
Specification MEF
Treatment with/without IR 20Gy 1hr
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 25 2015

Application IHC - Wholemount
Sample Mouse Embryo (stage e6.5)
Specification stage e6.5
Username

Abcam user community

Verified customer

提交于 Dec 15 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Human Cell (U2OS osteosarcoma)
Specification U2OS osteosarcoma
Permeabilization Yes - 0.5% Triton CSK buffer pre-extraction
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 31 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (6%)
Sample Human Cell lysate - whole cell (U2OS osteosarcoma)
Specification U2OS osteosarcoma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Mar 31 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (cervical cancer)
Specification cervical cancer
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 02 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (Hek293, MCF-7 and rat Vascular Smooth Muscle Cells)
Loading amount 20 µg
Specification Hek293, MCF-7 and rat Vascular Smooth Muscle Cells
Gel Running Conditions Reduced Denaturing (5% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Sep 22 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"