概述

  • 产品名称Anti-MDC抗体
    参阅全部 MDC 一抗
  • 描述
    兔多克隆抗体to MDC
  • 经测试应用适用于: Sandwich ELISA, Neutralising, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Highly pure (>98%) recombinant hMDC(human Macrophage-Derived Chemokine)

性能

  • 形式Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型unknown
  • 轻链类型unknown
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab9847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml.

This antibody used as the capture antibody can be paired with ab83129 as the detection antibody allowing the detection of at least 0.2 - 0.4 ng/well of recombinant hMDC.

Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hMDC (100 ng/ml), a concentration of 5.0 - 7.0 µg/ml of this antibody is required.
WB Use a concentration of 0.1 - 0.2 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 19318474

靶标

  • 功能May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active.
  • 组织特异性Highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. Also found in lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression in lung and spleen. Very weak expression in small intestine. In lymph node expressed in a mature subset of Langerhans' cells (CD1a+ and CD83+). Expressed in Langerhans' cell histiocytosis but not in dermatopathic lymphadenopathy. Expressed in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis.
  • 序列相似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻译后修饰The N-terminal processed forms MDC(3-69), MDC(5-69) and MDC(7-69) are produced by proteolytic cleavage after secretion from monocyte derived dendrocytes.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A 152E5.1 antibody
    • ABCD 1 antibody
    • ABCD1 antibody
    • C C motif chemokine 22 antibody
    • CC chemokine STCP 1 antibody
    • CC chemokine STCP-1 antibody
    • ccl 22 antibody
    • Ccl22 antibody
    • CCL22_HUMAN antibody
    • Chemokine (C C motif) ligand 22 antibody
    • DC/B CK antibody
    • DCBCK antibody
    • Macrophage-derived chemokine antibody
    • MDC antibody
    • MDC(1-69) antibody
    • MDC(7-69) antibody
    • MGC34554 antibody
    • SCYA22 antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 22 antibody
    • Small inducible cytokine subfamily A, member 22 antibody
    • Small-inducible cytokine A22 antibody
    • STCP 1 antibody
    • STCP1 antibody
    • Stimulated T cell chemotactic protein 1 antibody
    • Stimulated T-cell chemotactic protein 1 antibody
    see all

Anti-MDC antibody (ab9847)参考文献

This product has been referenced in:
  • Döring C  et al. A novel immunohistochemical classifier to distinguish Hodgkin lymphoma from ALK anaplastic large cell lymphoma. Mod Pathol N/A:N/A (2014). Read more (PubMed: 24633193) »
  • Li YQ  et al. Tumor Secretion of CCL22 Activates Intratumoral Treg Infiltration and Is Independent Prognostic Predictor of Breast Cancer. PLoS One 8:e76379 (2013). IHC-P ; Human . Read more (PubMed: 24124553) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Colorectal)
Permeabilization Yes - 1x PBS with 0.3% triton-x-100
Specification Colorectal
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Formaldehyde
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提交于 May 08 2015



ab9675 and ab9847 are not conjugated. If you would like to conjugate them to fluorochromes, we have easy kits which would allow you to do this. Please see: http://www.abcam.com/easylink

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"