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Our Abpromise guarantee covers the use of ab9847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml.
This antibody used as the capture antibody can be paired with ab83129 as the detection antibody allowing the detection of at least 0.2 - 0.4 ng/well of recombinant hMDC.
|Neutralising||Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hMDC (100 ng/ml), a concentration of 5.0 - 7.0 µg/ml of this antibody is required.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml.|
|IHC-P||Use at an assay dependent concentration. PubMed: 19318474|