概述

性能

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应用

Our Abpromise guarantee covers the use of ab4544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
ELISA
  • 应用说明Peptide ELISA: Antibody detection limit dilution >1:32,000.
    IHC-P: Use at a concentration of 10 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能RNA helicase that, through its ATP-dependent unwinding of RNA, may function to promote message degradation by specific RNases. Seems to have growth suppressive properties. Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). ATPase activity is specifically induced by dsRNA. Essential for the production of interferons in response to picornaviruses.
    • 组织特异性Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung.
    • 疾病相关Genetic variation in IFIH1 is associated with diabetes mellitus insulin-dependent type 19 (IDDM19) [MIM:610155]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
      Note=IFIH1 is the CADM-140 autoantigen, involved in clinically amyopathic dermatomyositis (CADM). This is a chronic inflammatory disorder that shows typical skin manifestations of dermatomyositis but has no or little evidence of clinical myositis. Anti-CADM-140 antibodies appear to be specific to dermatomyositis, especially CADM. Patients with anti-CADM-140 antibodies frequently develop life-threatening acute progressive interstitial lung disease (ILD).
    • 序列相似性Belongs to the helicase family.
      Contains 2 CARD domains.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • 翻译后修饰During apoptosis, processed into 3 cleavage products. The helicase-containing fragment, once liberated from the CARD domains, translocate from the cytoplasm to the nucleus. The processed protein significantly sensitizes cells to DNA degradation.
    • 细胞定位Cytoplasm. Nucleus. May be found in the nucleus, during apoptosis.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CADM-140 autoantigen antibody
      • Clinically amyopathic dermatomyositis autoantigen 140 kDa antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide antibody
      • DEAD/H box polypeptide antibody
      • Helicard antibody
      • Helicase with 2 CARD domains antibody
      • Hlcd antibody
      • IDDM 19 antibody
      • IDDM19 antibody
      • IFIH 1 antibody
      • Ifih1 antibody
      • IFIH1_HUMAN antibody
      • Interferon induced helicase C domain containing protein 1 antibody
      • interferon induced with helicase C domain 1 antibody
      • Interferon induced with helicase C domain protein 1 antibody
      • Interferon-induced helicase C domain-containing protein 1 antibody
      • Interferon-induced with helicase C domain protein 1 antibody
      • MDA 5 antibody
      • MDA-5 antibody
      • Melanoma differentiation associated protein 5 antibody
      • Melanoma differentiation-associated gene 5 antibody
      • Melanoma differentiation-associated protein 5 antibody
      • MGC133047 antibody
      • Murabutide down regulated protein antibody
      • Murabutide down-regulated protein antibody
      • RH 116 antibody
      • RH116 antibody
      • RIG I like receptor 2 antibody
      • RLR 2 antibody
      • RNA helicase DEAD box protein 116 antibody
      • RNA helicase-DEAD box protein 116 antibody
      see all

    Anti-MDA5 antibody 图像

    • Ab4544 (10ug/ml) staining human MDA5 in human pancreas by immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

      Ab4544 shows strong cytoplasmic staining of some cells in the islets of Langerhans.

    Anti-MDA5 antibody (ab4544)参考文献

    This product has been referenced in:
    • Sato S  et al. RNA helicase encoded by melanoma differentiation-associated gene 5 is a major autoantigen in patients with clinically amyopathic dermatomyositis: Association with rapidly progressive interstitial lung disease. Arthritis Rheum 60:2193-200 (2009). WB . Read more (PubMed: 19565506) »

    See 1 Publication for this product

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