概述

  • 产品名称Anti-MD2抗体
    参阅全部 MD2 一抗
  • 描述
    兔多克隆抗体to MD2
  • 经测试应用适用于: Flow Cyt, IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to amino acids near the middle region of human MD2, between residues 110 and 160.

  • 阳性对照
    • Mouse spleen lysate, rat spleen cells.

性能

应用

Our Abpromise guarantee covers the use of ab24182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/1000.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 18 kDa).Can be blocked with Human MD2 peptide (ab39815).
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both MD2 and TLR4, but not TLR4 alone, respond to LPS.
  • 翻译后修饰N-glycosylated; high-mannose.
  • 细胞定位Secreted > extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ESOP 1 antibody
    • ESOP-1 antibody
    • ESOP1 antibody
    • LY 96 antibody
    • Ly-96 antibody
    • LY96 antibody
    • LY96_HUMAN antibody
    • Lymphocyte antigen 96 antibody
    • md 2 antibody
    • MD 2 protein antibody
    • MD2 protein antibody
    • Myeloid differentiation protein 2 antibody
    • Protein MD 2 antibody
    • Protein MD-2 antibody
    • Protein MD2 antibody
    see all

Anti-MD2 antibody 图像

  • Anti-MD2 antibody (ab24182) at 1 µg/ml (mouse spleen lysate)

    Predicted band size : 18 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
  • ab24182 at 2µg/ml staining of MD2 in rat spleen cells by IHC

  •     ab 24182 at a 1/100 dilution staining Rat mixed primary glial cell culture by FACS analysis. The antibody was incubated with the cells for 45 minutes and then detected using an Alexa-Fluor ® 488 Goat anti-rabbit antibody. For the negative control, primary antibody was omitted.

    This image is courtesy of an Abreview by Nancy Nutile-McMenemy submitted on 16 March 2006.

    See Abreview

  • ab24182 at a 1/200 dilution staining rat spinal cord tissue sections from a 4% PFA transcardially perfused animal by Immunohistochemistry (Frozen sections). The tissue was paraformaldehyde fixed and incubated with the antibody for 18 hours. Bound antibody was detected using an HRP conjugated goat anti-mouse polyclonal antibody.

    This image is courtesy of an Abreview submitted by Nancy Nutile-McMenemy.

    See Abreview

  • All lanes : Anti-MD2 antibody (ab24182) at 2 µg/ml (in 5% milk in TBS/T 0.1% (blocking buffer) for 12 hours at 4°C)

    Lane 1 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours
    Lane 2 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours and LPS (50 ng/ml) for 10 minutes
    Lane 3 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours and ethanol (50 mM) for 10 minutes
    Lane 4 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours
    Lane 5 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours and LPS (50 ng/ml) for 10 minutes
    Lane 6 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours and ethanol (50 mM) for 10 minutes

    Lysates/proteins at 40 µg per lane.

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an Abreview submitted by C. Guerri

    Blocking Step: 5% Milk for 1 hour at room temperature.

    See Abreview

  • Flow cytometry analysis of Human corneal epithelial cell lines, HCEC and HCET, staining MD2 with ab24182.

    Cells were either unstimulated (unstim - black line) or stimulated (+IFN-γ - grey histogram), fixed in paraformaldehyde and incubated with primary antibody for 45 minutes at 4°C. A fluorescent-conjugated secondary antibody was used to detect staining.

Anti-MD2 antibody (ab24182)参考文献

This product has been referenced in:
  • Guo J  et al. LPS/TLR4-mediated stromal cells acquire an invasive phenotype and are implicated in the pathogenesis of adenomyosis. Sci Rep 6:21416 (2016). IHC-P ; Human . Read more (PubMed: 26898650) »
  • Guijarro-Muñoz I  et al. Lipopolysaccharide activates Toll-like receptor 4 (TLR4)-mediated NF-?B signaling pathway and proinflammatory response in human pericytes. J Biol Chem 289:2457-68 (2014). WB ; Human . Read more (PubMed: 24307174) »

See all 12 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (LIVER)
Specification LIVER
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Nov 17 2014

Abreviews
Application Western blot
Loading amount 20 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10)
Sample Rabbit Cell lysate - whole cell (primary microglia)
Specification primary microglia
Treatment 100ng/ml LPS for 24hours
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Miss. Jae Ok SEO

Verified customer

提交于 Apr 28 2014

Thank you for contacting us.

I would recommend ab24182. Of the anti- MD2 antibodies on our catalog, this is the most characterized, with thirteen Abreviews and numerous images. This is the only anti-MD2 product we currently offer which has b...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (kindey (liver transplatation 24h))
Specification kindey (liver transplatation 24h)
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate EDTA buffer, PH 6.2
Permeabilization No
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jun 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate EDTA buffer, PH 6.2
Permeabilization No
Blocking step protein block(Dako) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jun 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate EDTA buffer pH=6.2
Permeabilization No
Blocking step Dako Protein block(X0909) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jun 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (spleen)
Specification spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate EDTA buffer pH=6.2
Permeabilization No
Blocking step Dako Protein block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jun 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (liver)
Loading amount 15 µg
Specification liver
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jan 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (GI-tract - sigma)
Specification GI-tract - sigma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: DAKO antigen retrieval buffer pH6
Permeabilization No
Username

Dr. Ilka Werner-Martini

Verified customer

提交于 Oct 12 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (cortical astrocytes)
Loading amount 40 µg
Specification cortical astrocytes
Treatment siRNA MD-2 for 96h and stimulation with LPS(50ng/ml) and ethanol(50mM)for 10 min
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Consuelo Guerri

Verified customer

提交于 Jul 21 2010

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"