概述

  • 产品名称Anti-MCU抗体
  • 描述
    兔多克隆抗体to MCU
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Recombinant fragment: HHRTVHQRIA SWQNLGAVYC STVVPSDDVT VVYQNGLPVI SVRLPSRRER CQFTLKPISD SVGVFLRQLQ EEDRGIDRVA IYSPDGVRVA ASTGIDLLLL DDFKLV, corresponding to N terminal amino acids 47-152 of Human MCU (Uniprot ID = Q8NE86)

  • 阳性对照
    • Human duodenum tissue. Human cell line U-2 OS.

性能

应用

Our Abpromise guarantee covers the use of ab121499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Recommended fixative: PFA/Triton X-100

靶标

Anti-MCU antibody 图像

  • Immunohistochemical analysis of paraffin-embedded Human duodenum labeling MCU in glandular cells with ab121499 at 1/50 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 was performed.
  • Immunofluorescent staining of Human cell line U-2 OS (fixed and permeabilized in PFA/Triton X-100) labeling MCU with ab121499 at 4µg/ml.

Anti-MCU antibody (ab121499)参考文献

This product has been referenced in:
  • Rimessi A  et al. Mitochondrial Ca2+-dependent NLRP3 activation exacerbates the Pseudomonas aeruginosa-driven inflammatory response in cystic fibrosis. Nat Commun 6:6201 (2015). Read more (PubMed: 25648527) »
  • Das S  et al. miR-181c Regulates the Mitochondrial Genome, Bioenergetics, and Propensity for Heart Failure In Vivo. PLoS One 9:e96820 (2014). WB ; Rat . Read more (PubMed: 24810628) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Spinal Cord)
Permeabilization Yes - 0.3% TritonX-100
Specification Spinal Cord
Blocking step BSA + Normal Goat Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 07 2015

Abreviews
Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (4-12& BIS-TRIS)
Sample Human Cell lysate - whole cell (fibroblasts)
Specification fibroblasts
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Feb 20 2014

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Heart)
Loading amount 30 µg
Specification Heart
Treatment miRNA overexpression
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 27°C
Username

Dr. Samarjit Das

Verified customer

提交于 Apr 19 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"