Anti-MCP5抗体(ab9737)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to MCP5
  • 经测试应用
    适用于: WB, ELISA, Neutralisingmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Highly pure (>98%) recombinant mMCP-5 (mouse Macrophage/Monocyte Chemotactic Protein-5).

性能

  • 形式
    Lyophilised:Reconstitute with 200µl of sterile water.
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    unknown
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
Neutralising
  • 应用说明
    Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mMCP-5 (100.00 ng/ml), a concentration of 2.0 - 3.0 µg/ml of this antibody is required.

    ELISA:To detect mMCP-5 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMCP-5.

    Western Blot:To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • 靶标

    • 功能
      Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.
    • 组织特异性
      Predominantly expressed in the lymph nodes and thymus. Also found in the salivary glands containing lymph nodes, breast, heart, lung, brain, small intestine, kidney and colon.
    • 序列相似性
      Belongs to the intercrine beta (chemokine CC) family.
    • 细胞定位
      Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C-C motif chemokine 12 antibody
      • Ccl12 antibody
      • CCL12_MOUSE antibody
      • Macrophage/monocyte Chemotactic Protein antibody
      • MCP-1-related chemokine antibody
      • MCP-5 antibody
      • Monocyte chemoattractant protein 5 antibody
      • Monocyte chemotactic protein 5 antibody
      • OTTMUSP00000000792 antibody
      • Scya12 antibody
      • Small inducible cytokine A12 antibody
      • Small-inducible cytokine A12 antibody
      see all

    实验方案

    Anti-MCP5 antibody (ab9737)参考文献

    ab9737 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting Abcam. I am sorry that you are having trouble with ab9737. From what I have read about MCP5, it does have a predicted molecular weight of ~12kDa. I have a few questions/suggestions about the protocol that you are using: 1 - Doe...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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