概述

性能

  • 形式
    Lyophilised:Reconstitute with 200µl of sterile water.
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    unknown
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明
    ELISA:To detect mMCP-3 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMCP-3.

    Western Blot:To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    • 靶标

    • 功能
      Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
    • 序列相似性
      Belongs to the intercrine beta (chemokine CC) family.
    • 翻译后修饰
      O-glycosylated.
    • 细胞定位
      Secreted.
    • Information by UniProt
    • 数据库链接
      • 别名
        • C-C motif chemokine 7 antibody
        • Ccl7 antibody
        • CCL7_HUMAN antibody
        • Chemokine CC motif ligand 7 antibody
        • FIC antibody
        • MARC antibody
        • MCP-3 antibody
        • Monocyte chemoattractant protein 3 antibody
        • Monocyte chemotactic protein 3 antibody
        • NC28 antibody
        • RP23-350G1.4 antibody
        • SCYA6 antibody
        • SCYA7 antibody
        • Small-inducible cytokine A7 antibody
        see all

      实验方案

      文献

      This product has been referenced in:
      • Buerki C  et al. Functional relevance of novel p300-mediated lysine 314 and 315 acetylation of RelA/p65. Nucleic Acids Res 36:1665-80 (2008). WB ; Mouse . Read more (PubMed: 18263619) »

      See 1 Publication for this product

      发表研究结果有使用 ab9911？请让我们知道，以便我们可以引用本数据表中的参考文章。

      客户评价及客户问答

      提交评价 提交问题

      2 reviews or Q&A

      10
      Votes: Highest First

      Attached are the results of ab9911 on mouse tissues.  If MCP3 here is in the form of dimmer, that would be right size.   Any thoughts?

      Thank you for sending me your western blot data. That antibody does appear very specific. However I think its supposed to be a monomer. Have you seen any publications showing western blot data of MCP3? Also, from what mouse tissue is ...

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      I had a question about your Anti-Murine MCP-3 antibody. Can it be used for immunohistochemistry (specifically paraffin sections) and if so what is the optimal concentration?

      Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you ...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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