Anti-MCP3抗体[542] (ab18693)

概述

  • 产品名称
    Anti-MCP3抗体[542]
    参阅全部 MCP3 一抗
  • 描述
    小鼠单克隆抗体[542] to MCP3
  • 特异性
    This monoclonal antibody reacts with natural and recombinant human MCP 3. It does not show any cross reaction with other human cytokines or growth factors tested such as IL 1 beta, IL 8, MCAF, SAA and EGF.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Recombinant fragment (Human).(M.W. 8.5 kDa containing 76 amino acid residues).

  • 阳性对照
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: None
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    542
  • 骨髓瘤
    Sp2/0-Ag14
  • 同种型
    IgG2a
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab18693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 8.5 kDa.
    This antibody can be used as a capture antibody in sandwich ELISA applications for human MCP-3 detection in combination with a monoclonal tracer/detection antibody (HRP conjugated). Suggested Capture Coating Dose: 3µg/well; Substrate:TMB.
    A Western blot using an antibody concentration of 0.5-2µg/mL will allow visualization of 0.1µg/lane of human MCP-3.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
    • 序列相似性
      Belongs to the intercrine beta (chemokine CC) family.
    • 翻译后修饰
      O-glycosylated.
    • 细胞定位
      Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C-C motif chemokine 7 antibody
      • Ccl7 antibody
      • CCL7_HUMAN antibody
      • Chemokine CC motif ligand 7 antibody
      • FIC antibody
      • MARC antibody
      • MCP-3 antibody
      • Monocyte chemoattractant protein 3 antibody
      • Monocyte chemotactic protein 3 antibody
      • NC28 antibody
      • RP23-350G1.4 antibody
      • SCYA6 antibody
      • SCYA7 antibody
      • Small-inducible cytokine A7 antibody
      see all

    实验方案

    Anti-MCP3 antibody [542] (ab18693)参考文献

    ab18693 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Skin)
    Loading amount
    15 µg
    Specification
    Skin
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5%
    Username

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    Verified customer

    提交于 Apr 27 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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