概述

  • 产品名称Anti-MCP1抗体
    参阅全部 MCP1 一抗
  • 描述
    兔多克隆抗体to MCP1
  • 经测试应用适用于: ELISA, Neutralising, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Recombinant fragment, corresponding to amino acids 24/148 of Mouse MCP1(Peptide available as ab9901.)

  • 常规说明


    JE, also known as monocyte chemotactic protein (MCP-1), plays an important role in the inflammatory response of blood monocytes and tissue macrophages

性能

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应用

Our Abpromise guarantee covers the use of ab9899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration. To detect mJE by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mJE.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of mJE (100 ng/ml), a concentration of 4.0 - 6.0 µg/ml of this antibody is required.
WB Use at an assay dependent concentration. Can be blocked with Recombinant mouse MCP1 protein (ab9901). To detect mJE by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mJE is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • 序列相似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻译后修饰Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C-C motif chemokine 2 antibody
    • CCL2 antibody
    • CCL2_HUMAN antibody
    • Chemokine (C C motif) ligand 2 antibody
    • GDCF 2 antibody
    • GDCF-2 antibody
    • GDCF2 antibody
    • HC11 antibody
    • HSMCR30 antibody
    • JE antibody
    • MCAF antibody
    • MCP 1 antibody
    • MCP-1 antibody
    • MCP1 antibody
    • MGC9434 antibody
    • Monocyte chemoattractant protein 1 antibody
    • Monocyte chemotactic and activating factor antibody
    • Monocyte chemotactic protein 1 antibody
    • Monocyte secretory protein JE antibody
    • SCYA2 antibody
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
    • Small inducible cytokine A2 antibody
    • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
    • Small-inducible cytokine A2 antibody
    • SMC CF antibody
    • SMC-CF antibody
    • SMCCF antibody
    see all

Anti-MCP1 antibody 图像

  • staining in colchicine injected mouse brain (including caudate putamen) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein reagent. Optimal concentrations and conditions may vary.

Anti-MCP1 antibody (ab9899)参考文献

ab9899 has not yet been referenced specifically in any publications.

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