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Our Abpromise guarantee covers the use of ab9779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa. sed in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.|
|Neutralising||Use a concentration of 4 - 6 µg/ml. Yields one-half maximal inhibition [ND50] of the biological activity of Rat MCP1 (100 ng/ml).|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MCP1.|
|IHC-FoFr||Use at an assay dependent concentration.|