概述

  • 产品名称Anti-MCP1抗体
    参阅全部 MCP1 一抗
  • 描述
    兔多克隆抗体to MCP1
  • 特异性ab25124 reacts with both rat MCP1 and its mouse homolog JE.
  • 经测试应用适用于: IHC-P, Flow Cyt, Neutralising, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Recombinant fragment corresponding to Rat MCP1 aa 1-73 (N terminal).
    Sequence:

    MQVSVTLLGL LFTVAACSIH VLSQPDAVNA PLTCCYSFTG KMIPMSRLEN YKRITSSRCP KEAVVFVTKL KRE

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Rat Kidney.

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应用

Our Abpromise guarantee covers the use of ab25124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100.

Used at a dulition of 1/100 for 30 min on rat primary mixed cortical cells (see Abreview for further information).

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Neutralising Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 25 kDa.

靶标

  • 功能Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • 序列相似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻译后修饰Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C-C motif chemokine 2 antibody
    • CCL2 antibody
    • CCL2_HUMAN antibody
    • Chemokine (C C motif) ligand 2 antibody
    • GDCF 2 antibody
    • GDCF-2 antibody
    • GDCF2 antibody
    • HC11 antibody
    • HSMCR30 antibody
    • JE antibody
    • MCAF antibody
    • MCP 1 antibody
    • MCP-1 antibody
    • MCP1 antibody
    • MGC9434 antibody
    • Monocyte chemoattractant protein 1 antibody
    • Monocyte chemotactic and activating factor antibody
    • Monocyte chemotactic protein 1 antibody
    • Monocyte secretory protein JE antibody
    • SCYA2 antibody
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
    • Small inducible cytokine A2 antibody
    • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
    • Small-inducible cytokine A2 antibody
    • SMC CF antibody
    • SMC-CF antibody
    • SMCCF antibody
    see all

Anti-MCP1 antibody 图像

  • IHC image of MCP1 staining in Rat Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25124, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-MCP1 antibody (ab25124) at 1/1000 dilution

    Lane 1 : 40ug whole rat spinal cord protein lysate
    Lane 2 : 40ug whole mouse spinal cord protein lysate
    Lane 3 : Naive Rat
    Lane 4 : Sham 1
    Lane 5 : Sham 2
    Lane 6 : Injured 1
    Lane 7 : Injured 2
    Lane 8 : Injured 3

    Secondary
    Goat anti-Rabbit HRP
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes
    This image is courtesy of an Abreview by Nancy Nutile-McMenemy submitted on 7 December 2005.

    See Abreview

Anti-MCP1 antibody (ab25124)参考文献

This product has been referenced in:
  • Liu Z  et al. Mark4 promotes oxidative stress and inflammation via binding to PPAR? and activating NF-?B pathway in mice adipocytes. Sci Rep 6:21382 (2016). WB ; Mouse . Read more (PubMed: 26888669) »
  • Aoki K  et al. Role of Indoxyl Sulfate as a Predisposing Factor for Atrial Fibrillation in Renal Dysfunction. J Am Heart Assoc 4:e002023 (2015). WB, IHC . Read more (PubMed: 26452986) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer 1X
Permeabilization No
Specification kidney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 14 2015

Application Western blot
Sample Human Recombinant protein (Recombinant protein)
Loading amount 0.2 µg
Specification Recombinant protein
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Dec 17 2010

Thank you for your enquiry. We recommend that the azide be dialyzed out of the antibody preparation before any in vitro or in vivo studies.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Primary Mixed Cortical Cultures)
Specification Primary Mixed Cortical Cultures
Permeabilization No
Username

Ms. Nancy Nutile-McMenemy

Verified customer

提交于 Apr 12 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Spinal Cord)
Specification Spinal Cord
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Ms. Nancy Nutile-McMenemy

Verified customer

提交于 Dec 07 2005

Thank you for your enquiry. This antiserum was tested by western blotting using RAW cells treated with LPS for 15 hours. 10ug of lysate and 10ug of supernatant was loaded onto the gel. A prominent band of 25 kDa was detected. I hope this informat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"