The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.25µg for 105 cells. Use at less than or equal to 0.25 µg per test. A test is defined as the amount (µg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
ab101026-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
Belongs to the intercrine beta (chemokine CC) family.
Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
Flow cytometry staining of 24-hour LPS-stimulated normal Human peripheral blood cells with 0.25 µg of Armenian Hamster IgG Isotype Control PE (open histogram) or 0.25 µg of ab95558 (filled histogram). Cells in the monocyte gate were used for analysis.