概述

  • 产品名称Anti-MCM8抗体
    参阅全部 MCM8 一抗
  • 描述
    兔多克隆抗体to MCM8
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: LRDAPEKTLA CMGLAIHQVL TKDLERHAAE LQAQEGLSND GETMVNVPHI, corresponding to N terminal amino acids 145-194 of Human MCM8

  • 阳性对照
    • Jurkat cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab66761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.125 µg/ml. Detects a band of approximately 78, 82 kDa (predicted molecular weight: 93 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:62500.

靶标

  • 功能Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8-MCM9 complex probably acts as a hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart. May also play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC). Binds chromatin throughout the cell cycle.
  • 组织特异性Highest levels in placenta, lung and pancreas. Low levels in skeletal muscle and kidney. Expressed in various tumors with highest levels in colon and lung cancers.
  • 序列相似性Belongs to the MCM family.
    Contains 1 MCM domain.
  • 细胞定位Nucleus. Localizes to nuclear foci and colocalizes with RAD51.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C20orf154 antibody
    • dJ967N21.5 antibody
    • DNA helicase MCM8 antibody
    • DNA replication licensing factor MCM8 antibody
    • MCM8 antibody
    • MCM8_HUMAN antibody
    • MGC119522 antibody
    • MGC119523 antibody
    • MGC12866 antibody
    • MGC4816 antibody
    • Minichromosome maintenance 8 antibody
    • Minichromosome maintenance complex component 8 antibody
    • REC antibody
    see all

Anti-MCM8 antibody 图像

  • Anti-MCM8 antibody (ab66761) at 0.125 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 93 kDa
    Observed band size : 82 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 78 kDa. We are unsure as to the identity of these extra bands.

Anti-MCM8 antibody (ab66761)参考文献

ab66761 has not yet been referenced specifically in any publications.

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