Anti-MCM7/PRL抗体(ab96849)
Key features and details
- Rabbit polyclonal to MCM7/PRL
- Suitable for: ChIP, WB, IHC-P, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MCM7/PRL抗体
参阅全部 MCM7/PRL 一抗 -
描述
兔多克隆抗体to MCM7/PRL -
宿主
Rabbit -
经测试应用
适用于: ChIP, WB, IHC-P, ICC/IF, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Zebrafish -
免疫原
Recombinant fragment corresponding to Human MCM7/PRL aa 14-275.
Database link: P33993 -
阳性对照
- WB: H1299 cell lysate. IHC-P: Cal27 Xenograft tissue. ICC/IF: HeLa cells. IP: HeLa cells. ChIP: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96849于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ChIP |
Use at an assay dependent concentration.
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WB |
1/500 - 1/3000. Predicted molecular weight: 81 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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IP |
1/100 - 1/250.
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说明 |
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ChIP
Use at an assay dependent concentration. |
WB
1/500 - 1/3000. Predicted molecular weight: 81 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
IP
1/100 - 1/250. |
靶标
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功能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. -
序列相似性
Belongs to the MCM family.
Contains 1 MCM domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 539924 Cow
- Entrez Gene: 4176 Human
- Entrez Gene: 17220 Mouse
- Entrez Gene: 288532 Rat
- Omim: 600592 Human
- SwissProt: Q3ZBH9 Cow
- SwissProt: P33993 Human
- SwissProt: Q61881 Mouse
see all -
别名
- CDABP0042 antibody
- CDC 47 antibody
- CDC47 antibody
see all
图片
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Anti-MCM7/PRL antibody (ab96849) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 81 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using ab96849 at 1/200 dilution (1).Merged with DNA probe (2).
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Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft using ab96849 at 1/100 dilution.
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MCM7 was immunoprecipitated from 40 μg of HeLa whole cell lysate with ab96849 at 1/1000 dilution. EasyBlot anti-rabbit IgG, was used as secondary antibody.
Lane 1: 40 μg HeLa whole cell extract (Input).
Lane 2: Control with 4 μg of preimmune Rabbit IgG.
Lane 3: Immunoprecipitation of MCM7 protein by 4 μg MCM7 antibody [N2C2], Internal.7.5 % SDS-PAGE
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Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab96849 被引用在 1 文献中.
- Miotto B et al. The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability. Cell Rep 7:575-87 (2014). WB . PubMed: 24726359