概述

  • 产品名称Anti-MCM7抗体[47DC141]
    参阅全部 MCM7 一抗
  • 描述
    小鼠单克隆抗体[47DC141] to MCM7
  • 经测试应用适用于: IHC-P, IP, WB, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human, Xenopus laevis
  • 免疫原

    Recombinant full length protein (Human).

  • 阳性对照
    • Breast carcinoma, MAD109 cell lysate, PC12 cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号47DC141
  • 骨髓瘤unknown
  • 同种型IgG1
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. This is when using an ABC method for 30 minutes at room temperature. Sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
IP Use at 2 µg/mg of lysate.
WB 1/25 - 1/50. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa). Incubate for 2 hrs at RT for colorimetric detection, can dilute more with ECL+ and with overnight incubation. By Western blot, this antibody detects a band of 80 kDa, which corresponds to the predicted molecular weight of Cdc47 / MCM7.
Flow Cyt 1/20.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
  • 序列相似性Belongs to the MCM family.
    Contains 1 MCM domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDABP0042 antibody
    • CDC 47 antibody
    • CDC47 antibody
    • CDC47 homolog antibody
    • Cdc47, S. cerevisiae, homolog of antibody
    • DNA replication licensing factor MCM7 antibody
    • Homolog of S. cerevisiae Cdc47 antibody
    • MCM 2 antibody
    • MCM 7 antibody
    • MCM2 antibody
    • MCM2, formerly antibody
    • Mcm7 antibody
    • MCM7 minichromosome maintenance deficient 7 antibody
    • MCM7_HUMAN antibody
    • Minichromosome Maintainence 7 antibody
    • Minichromosome maintainence, S. cerevisiae, homolog of antibody
    • Minichromosome maintenance complex component 7 antibody
    • Minichromosome maintenance deficient 7 antibody
    • Minichromosome maintenance protein 7 antibody
    • P1.1 MCM3 antibody
    • P1.1-MCM3 antibody
    • P1CDC47 antibody
    • P85MCM antibody
    • PNAS 146 antibody
    • PNAS146 antibody
    see all

Anti-MCM7 antibody [47DC141] 图像

  • ab2360 - immunohistochemistry

    Formalin fixed paraffin embedded human tonsil stained with MCM7, using ABC and DAB chromagen.

  • This image shows immunostaining of rat brain endothelial cells. Brain endothelial cells were co-cultured with neuronal precursor cells and the nuclear staining represents cells in cell cycle. Primary antibody (ab2360) was used at 1:50 dilution, incubated overnight at 4 oC.  Secondary antibody - Alexafluor (488 nm) at 1:200 dilution, incubated for 2 hours at room temperature.

    The picture was kindly supplied by Dr Joseph Corteza Lim and Dr Margery Barrand from University of Cambridge, Department of Pharmacology.

     
     
  • All lanes : Anti-MCM7 antibody [47DC141] (ab2360) at 1/200 dilution

    Lane 1 : M phase Xenopus laevis egg extract, whole tissue lysate.
    Lane 2 : I phase Xenopus laevis egg extract, whole tissue lysate.

    Secondary
    HRP conjugated Donkey anti-rabbit IgG
    Developed using the ECL technique

    Predicted band size : 80 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab2360 staining MCM7 in human bladder cancer tissue sections by Immunohistochemistry (formalin fixed sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Tissue was blocked with 5% BSA for 1 hour at room temperature followed by incubation with the primary antibody at a 1/1200 dilution for 1 hour. A HRP-conjugated goat anti-mouse polyclonal was used as secondary antibody un-diluted.

    See Abreview

  • Lane 1 : Anti-MCM7 antibody [47DC141] (ab2360) at 1/50 dilution
    Lane 2 : Anti-MCM7 antibody [47DC141] (ab2360) at 1/200 dilution
    Lane 3 : Anti-MCM7 antibody [47DC141] (ab2360) at 1/500 dilution

    Lane 1 : Whole cell lysate prepared from SW780 cells
    Lane 2 : Whole cell lysate prepared from SW780 cells
    Lane 3 : Whole cell lysate prepared from SW780 cells

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG conjugated to HRP at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Observed band size : 81 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 minutes

    Image kindly supplied by Dr Karin Birkenkamp-Demtroeder through Abreview

    Gel run under denaturing conditions 4-12% gradient.

    See Abreview

  • ICC/IF image of ab2360 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2360, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLA cells stained with ab2360 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2360, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab2360 staining MCM7 in formalin-fixed, paraffin-embedded Human breast carcinoma tissue tissue by Immunohistochemistry.

Anti-MCM7 antibody [47DC141] (ab2360)参考文献

This product has been referenced in:
  • Bai G  et al. Chronic DNA Replication Stress Reduces Replicative Lifespan of Cells by TRP53-Dependent, microRNA-Assisted MCM2-7 Downregulation. PLoS Genet 12:e1005787 (2016). WB ; Mouse . Read more (PubMed: 26765334) »
  • Renaudin X  et al. Proteomic analysis reveals a FANCA-modulated neddylation pathway involved in CXCR5 membrane targeting and cell mobility. J Cell Sci 127:3546-54 (2014). Read more (PubMed: 25015289) »

See all 13 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (3T3MEF)
Gel Running Conditions Non-reduced Denaturing (7.5% acrylamide-Bis gel)
Loading amount 50000 cells
Treatment by serum starvation for 48h , cells are synchronized at G0 phase and released with 5% FBS and collected at indicated time
Specification 3T3MEF
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Dec 18 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA-Buffer pH 9,0
Sample Human Tissue sections (Tonsil)
Specification Tonsil
Permeabilization Yes - with wash - Buffer with Tween
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

提交于 Jun 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (SW780 bladder cancer)
Loading amount 25 µg
Specification SW780 bladder cancer
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 19°C
Username

Dr. Karin Birkenkamp-Demtroeder

Verified customer

提交于 May 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Bladder Cancer / Transitional cell Carcinoma)
Specification Bladder Cancer / Transitional cell Carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Karin Birkenkamp-Demtroeder

Verified customer

提交于 Apr 29 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T (LANE 1 AND 2))
Loading amount 25 µg
Specification 293T (LANE 1 AND 2)
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 31 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Tissue lysate - whole (Egg extracts)
Loading amount 12 µg
Specification Egg extracts
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Jul 25 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"