概述

  • 产品名称Anti-MCM6抗体
    参阅全部 MCM6 一抗
  • 描述
    兔多克隆抗体to MCM6
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide (Human) - 31 amino acids which represented a portion of human MCM 6 encoded by exon 17.

性能

应用

Our Abpromise guarantee covers the use of ab4458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 91 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
    • 序列相似性Belongs to the MCM family.
      Contains 1 MCM domain.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus. Binds to chromatin during G1 and detach from it during S phase.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DNA replication licensing factor MCM6 antibody
      • MCG40308 antibody
      • Mcm6 antibody
      • MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) antibody
      • MCM6 minichromosome maintenance deficient 6 antibody
      • MCM6_HUMAN antibody
      • Minichromosome maintenance complex component 6 antibody
      • Minichromosome maintenance deficient (mis5 S. pombe) 6 antibody
      • Minichromosome maintenance deficient 6 homolog antibody
      • Minichromosome maintenance protein 6 antibody
      • Mis5 antibody
      • MIS5 homolog antibody
      • p105MCM antibody
      see all

    Anti-MCM6 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling MCM6 with ab4458 at 1/1000 (1µg/ml). Detection: DAB.


    • Predicted band size : 91 kDa

      Sample: HeLa cell RIPA extract (50 µg).

      Antibody: ab4458 at 0.2 µg/ml incubated for 1 hour.

      Sample: HeLa cell RIPA extract (50 µg).

      Antibody: ab4458 at 0.2 µg/ml incubated for 1 hour.
    • IHC image of ab4458 staining in normal human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4458, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-MCM6 antibody (ab4458)参考文献

    This product has been referenced in:
    • Quimbaya M  et al. Deregulation of the Replisome Factor MCMBP Prompts Oncogenesis in Colorectal Carcinomas through Chromosomal Instability. Neoplasia 16:694-709 (2014). Human . Read more (PubMed: 25246271) »
    • Kawabata T  et al. A reduction of licensed origins reveals strain-specific replication dynamics in mice. Mamm Genome 22:506-17 (2011). WB ; Mouse . Read more (PubMed: 21611832) »

    See all 3 Publications for this product

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    Thanks for your enquiry. Unfortunately, we are not aware of any publications that feature the use of either of these antibodies. We also have not received any feedback from customers in the form of reviews who have used these antibodies. For both...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"