概述

  • 产品名称Anti-MCM6抗体
    参阅全部 MCM6 一抗
  • 描述
    山羊多克隆抗体to MCM6
  • 经测试应用适用于: IHC-P, WB, IHC-Frmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide (Human) - which represented a portion of human Minichromosome Maintenance Deficient 6 encoded by exon 17.

  • 阳性对照
    • HeLa cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab3730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 21980527

靶标

  • 功能Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
  • 序列相似性Belongs to the MCM family.
    Contains 1 MCM domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Binds to chromatin during G1 and detach from it during S phase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA replication licensing factor MCM6 antibody
    • MCG40308 antibody
    • Mcm6 antibody
    • MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) antibody
    • MCM6 minichromosome maintenance deficient 6 antibody
    • MCM6_HUMAN antibody
    • Minichromosome maintenance complex component 6 antibody
    • Minichromosome maintenance deficient (mis5 S. pombe) 6 antibody
    • Minichromosome maintenance deficient 6 homolog antibody
    • Minichromosome maintenance protein 6 antibody
    • Mis5 antibody
    • MIS5 homolog antibody
    • p105MCM antibody
    see all

Anti-MCM6 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MCM6 with ab3730 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 115 kDa

    Sample: Hela cell RIPA extract (60µg).

    Antibody: Affinity purified Goat anti-MCM 6 (ab3730) diluted 1 / 4000.

    Detection: ECL.

     

    Sample: Hela cell RIPA extract (60µg).

    Antibody: Affinity purified Goat anti-MCM 6 (ab3730) diluted 1 / 4000.

    Detection: ECL.

  • Immunofluorescence analysis of rat kidney tissue collected at 0 hours (shame) and 24 hours after ischemic injury, and embryonic rat kidney tissue from embryonic day 17 (E17d).

    MCM6 (red) was stained using ab3730 at 1/1000 dilution. Nuclei were stained with DAPI (blue).

Anti-MCM6 antibody (ab3730)参考文献

This product has been referenced in:
  • Kim TM  et al. Gene expression analysis reveals the cell cycle and kinetochore genes participating in ischemia reperfusion injury and early development in kidney. PLoS One 6:e25679 (2011). IHC-Fr ; Rat . Read more (PubMed: 21980527) »

See 1 Publication for this product

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