- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-MCM6抗体
参阅全部 MCM6 一抗 -
描述山羊多克隆抗体to MCM6
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宿主Goat
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经测试应用适用于: IHC-P, WB, IHC-Frmore details
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种属反应性与反应: Rat, Human
预测可用于: Mouse, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide (Human) - which represented a portion of human Minichromosome Maintenance Deficient 6 encoded by exon 17.
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阳性对照
- HeLa cell lysate.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab3730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/2000 - 1/10000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa). | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 21980527 |
靶标
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功能Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
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序列相似性Belongs to the MCM family.
Contains 1 MCM domain. -
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
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细胞定位Nucleus. Binds to chromatin during G1 and detach from it during S phase.
- Information by UniProt
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数据库链接
- Entrez Gene: 517812 Cow
- Entrez Gene: 4175 Human
- Entrez Gene: 17219 Mouse
- Entrez Gene: 29685 Rat
- Omim: 601806 Human
- SwissProt: Q2KIZ8 Cow
- SwissProt: Q14566 Human
- SwissProt: P97311 Mouse
see all -
别名
- DNA replication licensing factor MCM6 antibody
- MCG40308 antibody
- Mcm6 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (ab3730)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MCM6 with ab3730 at 1/1000 (1µg/ml). Detection: DAB. -
Western blot - Anti-MCM6 antibody (ab3730)Sample: Hela cell RIPA extract (60
µ g).Antibody: Affinity purified Goat anti-MCM 6 (ab3730) diluted 1 / 4000.
Detection: ECL.
Sample: Hela cell RIPA extract (60
µg). Antibody: Affinity purified Goat anti-MCM 6 (ab3730) diluted 1 / 4000.
Detection: ECL.
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Immunohistochemistry (Frozen sections) - Anti-MCM6 antibody (ab3730)Image from Kim TM et al., PLoS One. 2011;6(9):e25679. Epub 2011 Sep 28. Fig 6.; doi:10.1371/journal.pone.0025679; September 28, 2011, PLoS ONE 6(9): e25679.Immunofluorescence analysis of rat kidney tissue collected at 0 hours (shame) and 24 hours after ischemic injury, and embryonic rat kidney tissue from embryonic day 17 (E17d).
MCM6 (red) was stained using ab3730 at 1/1000 dilution. Nuclei were stained with DAPI (blue).
文献
This product has been referenced in:
- Kim TM et al. Gene expression analysis reveals the cell cycle and kinetochore genes participating in ischemia reperfusion injury and early development in kidney. PLoS One 6:e25679 (2011). IHC-Fr ; Rat . Read more (PubMed: 21980527) »
客户评价及客户问答
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