Synthetic peptide (Human) - which represented a portion of human Minichromosome Maintenance Deficient 6 encoded by exon 17.
Our Abpromise guarantee covers the use of ab3730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
WB | 1/2000 - 1/10000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa). | |
IHC-Fr | Use at an assay dependent concentration. PubMed: 21980527 |
Sample: Hela cell RIPA extract (60
Antibody: Affinity purified Goat anti-MCM 6 (ab3730) diluted 1 / 4000.
Detection: ECL.
Sample: Hela cell RIPA extract (60
Antibody: Affinity purified Goat anti-MCM 6 (ab3730) diluted 1 / 4000.
Detection: ECL.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"