Immunofluorescence staining of MCF7 cells with purified ab75975 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.
Western blot - MCM5 antibody [EP2683Y] (ab75975)
All lanes : Anti-MCM5 antibody [EP2683Y] (ab75975) at 1/10000 dilution
Lane 1 : HeLa lysate Lane 2 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 82 kDa Observed band size : 82 kDa
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