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Our Abpromise guarantee covers the use of ab6154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 82 kDa.|
|IHC-P||1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 18693245|
ab6154 staining MCM5 in the HeLa cell line from Human by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde , permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/200) for 1 hour at 22°C. ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed polyclonal was used as the secondary antibody (1/200).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"