重组Anti-MCM4抗体[EPR5245] (ab124836)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5245] to MCM4
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MCM4抗体[EPR5245]
参阅全部 MCM4 一抗 -
描述
兔单克隆抗体[EPR5245] to MCM4 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide within Human MCM4 aa 800-900 (C terminal). The exact sequence is proprietary.
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阳性对照
- HeLa, Molt-4, and A431 cell lysates and Molt-4 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.80 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5245 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124836于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/50000 - 1/200000. Predicted molecular weight: 97 kDa.
|
说明 |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/50000 - 1/200000. Predicted molecular weight: 97 kDa. |
靶标
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功能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. -
序列相似性
Belongs to the MCM family.
Contains 1 MCM domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4173 Human
- Entrez Gene: 17217 Mouse
- Omim: 602638 Human
- SwissProt: P33991 Human
- SwissProt: P49717 Mouse
- Unigene: 460184 Human
- Unigene: 1500 Mouse
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别名
- 19G antibody
- AI325074 antibody
- AU045576 antibody
see all
图片
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All lanes : Anti-MCM4 antibody [EPR5245] (ab124836) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Molt-4 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 97 kDa -
Intracellular flow cytometric analysis of permeabilized Molt-4 cells using anti-MCM4 in red (ab124836) or a rabbit IgG in green (negative).
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab124836 被引用在 2 文献中.
- Zhao Y et al. A Combined Treatment with Berberine and Andrographis Exhibits Enhanced Anti-Cancer Activity through Suppression of DNA Replication in Colorectal Cancer. Pharmaceuticals (Basel) 15:N/A (2022). PubMed: 35337060
- Zhao C et al. Quantitative proteomics using SILAC-MS identifies N-acetylcysteine-solution-triggered reversal response of renal cell carcinoma cell lines. J Cell Biochem 120:9506-9513 (2019). PubMed: 30520128