Anti-MCM2 (phospho S53)抗体(ab83969)

概述

  • 产品名称Anti-MCM2 (phospho S53)抗体
    参阅全部 MCM2 一抗
  • 描述
    兔多克隆抗体to MCM2 (phospho S53)
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic phosphorylated peptide corresponding to a portion of human MCM2 (NP_004517.2) surrounding serine 53.

  • 阳性对照
    • Human controls: breast carcinoma, colon adenocarcinoma, ovarian carcinoma, stomach adenocarcinoma and testicular seminoma. Mouse controls: squamous cell carcinoma.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab83969 was affinity purified using an epitope specific to MCM2 (phospho S53) immobilized on a solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明IHC-P: 1/100 - 1/500.
    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
    • 序列相似性Belongs to the MCM family.
      Contains 1 MCM domain.
    • 翻译后修饰Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BM28 antibody
      • CCNL 1 antibody
      • CCNL1 antibody
      • CDC like 1 antibody
      • cdc19 antibody
      • CDCL 1 antibody
      • CDCL1 antibody
      • Cell devision cycle like 1 antibody
      • Cyclin like 1 antibody
      • D3S3194 antibody
      • DNA replication licensing factor MCM2 antibody
      • KIAA0030 antibody
      • MCM 2 antibody
      • MCM2 antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
      • MCM2 minichromosome maintenance deficient 2, mitotin antibody
      • MCM2_HUMAN antibody
      • MGC10606 antibody
      • Minichromosome maintenance complex component 2 antibody
      • Minichromosome maintenance deficient 2 (mitotin) antibody
      • Minichromosome maintenance deficient 2 mitotin antibody
      • Minichromosome maintenance protein 2 antibody
      • Minichromosome maintenance protein 2 homolog antibody
      • Mitotin antibody
      • Nuclear protein BM28 antibody
      • OTTHUMP00000216047 antibody
      • OTTHUMP00000216050 antibody
      see all

    Anti-MCM2 (phospho S53) antibody 图像

    • ab83969, at a 1/500 dilution, staining MCM2 (phospho S53) in formaldehyde fixed, paraffin embedded human colon adenocarcinoma sections by Immunohistochemistry.
      Left image: mock phosphatase treated section
      Right image: calf intestinal phosphatase treated section.
      Detection: DAB staining using anti-rabbit IHC antibody at a dilution of 1/100.
    • ab83969, at a 1/500 dilution, staining MCM2 (phospho S53) in formaldehyde fixed, paraffin embedded mouse squamous cell carcinoma sections by Immunohistochemistry.
      Left image: mock phosphatase treated section
      Right image: calf intestinal phosphatase treated section.
      Detection: DAB staining using anti-rabbit IHC antibody at a dilution of 1/100.

    Anti-MCM2 (phospho S53) antibody (ab83969)参考文献

    ab83969 has not yet been referenced specifically in any publications.

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