重组Anti-MCM2 (phospho S27)抗体[EPR4119] (ab109459)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4119] to MCM2 (phospho S27)
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MCM2 (phospho S27)抗体[EPR4119]
参阅全部 MCM2 一抗 -
描述
兔单克隆抗体[EPR4119] to MCM2 (phospho S27) -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-P, ICC/IFmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa whole cell lysate (ab150035) and HeLa cells. Human cervical carcinoma tissue. Human tonsil tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4119 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109459于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 102 kDa.
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IP |
1/10 - 1/100.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
|
ICC/IF |
1/100 - 1/250.
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说明 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 102 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
ICC/IF
1/100 - 1/250. |
靶标
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功能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. -
序列相似性
Belongs to the MCM family.
Contains 1 MCM domain. -
翻译后修饰
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4171 Human
- Entrez Gene: 17216 Mouse
- Entrez Gene: 312538 Rat
- Omim: 116945 Human
- SwissProt: P49736 Human
- SwissProt: P97310 Mouse
- Unigene: 477481 Human
- Unigene: 16711 Mouse
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别名
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
图片
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All lanes : Anti-MCM2 (phospho S27) antibody [EPR4119] (ab109459) at 1/10000 dilution
Lane 1 : HeLa cell lysate untreated with alkaline phosphatase
Lane 2 : HeLa cell lysate treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa -
ab109459 at 1/100 dilution staining MCM2 (phospho S27) in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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MCM2 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) with ab109459 at 1/100 dilution.
Exposue time:10 second.
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ab109459 at 1/100 dilution staining MCM2 (phospho S27) in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab109459 at 1/100 dilution staining MCM2 (phospho S27) in HeLa cells by Immunofluorescence.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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