Anti-MCM2抗体[CRCT2.1] (ab81706)

概述

  • 产品名称Anti-MCM2抗体[CRCT2.1]
    参阅全部 MCM2 一抗
  • 描述
    小鼠单克隆抗体[CRCT2.1] to MCM2
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Bacterially expressed human MCM2

  • 表位Not determined
  • 阳性对照
    • HeLa cells. Tonsil

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号CRCT2.1
  • 同种型IgG1
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab81706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 102 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
  • 序列相似性Belongs to the MCM family.
    Contains 1 MCM domain.
  • 翻译后修饰Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BM28 antibody
    • CCNL 1 antibody
    • CCNL1 antibody
    • CDC like 1 antibody
    • cdc19 antibody
    • CDCL 1 antibody
    • CDCL1 antibody
    • Cell devision cycle like 1 antibody
    • Cyclin like 1 antibody
    • D3S3194 antibody
    • DNA replication licensing factor MCM2 antibody
    • KIAA0030 antibody
    • MCM 2 antibody
    • MCM2 antibody
    • MCM2 minichromosome maintenance deficient 2 mitotin antibody
    • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
    • MCM2 minichromosome maintenance deficient 2, mitotin antibody
    • MCM2_HUMAN antibody
    • MGC10606 antibody
    • Minichromosome maintenance complex component 2 antibody
    • Minichromosome maintenance deficient 2 (mitotin) antibody
    • Minichromosome maintenance deficient 2 mitotin antibody
    • Minichromosome maintenance protein 2 antibody
    • Minichromosome maintenance protein 2 homolog antibody
    • Mitotin antibody
    • Nuclear protein BM28 antibody
    • OTTHUMP00000216047 antibody
    • OTTHUMP00000216050 antibody
    see all

Anti-MCM2 antibody [CRCT2.1] 图像

  • Formalin fixed, paraffin embedded human tonsil tissue stained with anti MCM2 (form of ab81706 containing BSA and azide) at a dilution of 1/50. Peroxidase conjugate and AEC chromogen were used. Note nuclear staining of lymphocytes.
  • anti MCM2 (form of ab81706 containing BSA and azide) at 4 µg/ml + human cells

    Predicted band size : 102 kDa
    Observed band size : 102 kDa

Anti-MCM2 antibody [CRCT2.1] (ab81706)参考文献

ab81706 has not yet been referenced specifically in any publications.

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