概述

  • 产品名称
  • 描述
    山羊多克隆抗体to MCM2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human MCM2 aa 1-50.
    Database link: NP_0045117.2

  • 阳性对照
    • HeLa cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7
    Preservative: 0.09% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa).

靶标

  • 功能
    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
  • 序列相似性
    Belongs to the MCM family.
    Contains 1 MCM domain.
  • 翻译后修饰
    Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BM28 antibody
    • CCNL 1 antibody
    • CCNL1 antibody
    • CDC like 1 antibody
    • cdc19 antibody
    • CDCL 1 antibody
    • CDCL1 antibody
    • Cell devision cycle like 1 antibody
    • Cyclin like 1 antibody
    • D3S3194 antibody
    • DNA replication licensing factor MCM2 antibody
    • KIAA0030 antibody
    • MCM 2 antibody
    • MCM2 antibody
    • MCM2 minichromosome maintenance deficient 2 mitotin antibody
    • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
    • MCM2 minichromosome maintenance deficient 2, mitotin antibody
    • MCM2_HUMAN antibody
    • MGC10606 antibody
    • Minichromosome maintenance complex component 2 antibody
    • Minichromosome maintenance deficient 2 (mitotin) antibody
    • Minichromosome maintenance deficient 2 mitotin antibody
    • Minichromosome maintenance protein 2 antibody
    • Minichromosome maintenance protein 2 homolog antibody
    • Mitotin antibody
    • Nuclear protein BM28 antibody
    • OTTHUMP00000216047 antibody
    • OTTHUMP00000216050 antibody
    see all

Anti-MCM2 antibody 图像



  • Predicted band size : 120 kDa

    Sample: Hela cell RIPA extract (60µg).

    Antibody: Affinity purified Goat anti-MCM 2 (ab3723) diluted 1 / 4000.

    Detection: ECL.

    Sample: Hela cell RIPA extract (60µg). Antibody: Affinity purified Goat anti-MCM 2 (ab3723) diluted 1 / 4000. Detection: ECL.
  • All lanes : Anti-MCM2 antibody (ab3723) at 0.5 µg/ml

    Lane 1 : NIH3T3 cells
    Lane 2 : TCMK-1 cells
    Lane 3 : 4T1 cells
    Lane 4 : CT26.WT cells

    Lysates/proteins at 50 µg per lane.


    Predicted band size : 120 kDa

    Detection:Chemiluminescence with an exposed time of 3 minutes.

实验方案

Anti-MCM2 antibody (ab3723)参考文献

ab3723 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse Embryo (day 8.5))
Specification
Mouse Embryo (day 8.5)
Fixative
Paraformaldehyde
Permeabilization
Yes - Saponin
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
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Derek Passer

Verified customer

提交于 Aug 26 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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