The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000.
Use at 2-10 µg/mg of lysate.
Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion.
Belongs to the MCMBP family.
Nucleus. Associates with chromatin. Highly associated with chromatin in G1/S and S phases, reduced binding to chromatin in G2, and further decreased binding in early M phase. It then reassociates with chromatin in late M phase. Dissociates from chromatin later than component of the MCM complex.
ab124290 at 1 µg/ml staining MCM-BP in HeLa whole cell lysate immunoprecipitated using ab124290 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: control IgG.
Predicted band size : 73 kDa.
Exposure time: 30 seconds