概述

  • 产品名称Anti-MCM-BP抗体
    参阅全部 MCM-BP 一抗
  • 描述
    兔多克隆抗体to MCM-BP
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 150-200 of Human MCM-BP (NP_079110.1).

  • 阳性对照
    • HeLa, 293T and Jurkat whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab124290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 功能Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion.
  • 序列相似性Belongs to the MCMBP family.
  • 细胞定位Nucleus. Associates with chromatin. Highly associated with chromatin in G1/S and S phases, reduced binding to chromatin in G2, and further decreased binding in early M phase. It then reassociates with chromatin in late M phase. Dissociates from chromatin later than component of the MCM complex.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C10orf119 antibody
    • chromosome 10 open reading frame 119 antibody
    • MCM-binding protein antibody
    • MCM-BP antibody
    • mcmbp antibody
    • MCMBP_HUMAN antibody
    • Mini-chromosome maintenance complex-binding protein antibody
    • Minichromosome maintenance complex binding protein antibody
    see all

Anti-MCM-BP antibody 图像

  • All lanes : Anti-MCM-BP antibody (ab124290) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Exposure time : 3 minutes
  • ab124290 at 1 µg/ml staining MCM-BP in HeLa whole cell lysate immunoprecipitated using ab124290 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Predicted band size : 73 kDa. Exposure time: 30 seconds

Anti-MCM-BP antibody (ab124290)参考文献

ab124290 has not yet been referenced specifically in any publications.

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