Anti-MCL1 (phospho S159)抗体(ab111574)


  • 产品名称Anti-MCL1 (phospho S159)抗体
    参阅全部 MCL1 一抗
  • 描述
    兔多克隆抗体to MCL1 (phospho S159)
  • 特异性ab111574 detects endogenous levels of MCL1 only when phosphorylated at serine 159.
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic phosphopeptide derived from Human MCL1 around the phosphorylation site of Serine 159 (D-G-SP-L-P).

  • 阳性对照
    • A549 cells.



Our Abpromise guarantee covers the use of ab111574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 37 kDa.
ICC/IF 1/100 - 1/500.


  • 功能Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
  • 序列相似性Belongs to the Bcl-2 family.
  • 翻译后修饰Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
    Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
    Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
  • 细胞定位Membrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Bcl 2 related protein EAT/mcl1 antibody
    • Bcl-2-like protein 3 antibody
    • Bcl-2-related protein EAT/mcl1 antibody
    • BCL2 related antibody
    • Bcl2-L-3 antibody
    • BCL2L3 antibody
    • EAT antibody
    • Induced myeloid leukemia cell differentiation protein Mcl 1 antibody
    • Induced myeloid leukemia cell differentiation protein Mcl-1 antibody
    • MCL 1 antibody
    • MCL1 antibody
    • MCL1-ES antibody
    • mcl1/EAT antibody
    • MCL1_HUMAN antibody
    • MCL1L antibody
    • MCL1S antibody
    • MGC104264 antibody
    • MGC1839 antibody
    • Myeloid Cell Leukemia 1 antibody
    • Myeloid cell leukemia ES antibody
    • Myeloid cell leukemia sequence 1 antibody
    • Myeloid cell leukemia sequence 1 BCL2 related antibody
    • Myeloid cell leukemia sequence 1 isoform 1 antibody
    • OTTHUMP00000032794 antibody
    • OTTHUMP00000032795 antibody
    • TM antibody
    see all

Anti-MCL1 (phospho S159) antibody 图像

  • ab111574, at 1/100 dilution, staining MCL1 in A549 cells by Immunofluorescence, in the presence (right panel) or absence (left panel) of immunising peptide.

Anti-MCL1 (phospho S159) antibody (ab111574)参考文献

This product has been referenced in:

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Liver cells)
Loading amount 80 µg
Specification Liver cells
Treatment DMSO 24 h or tBHQ different time periods
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

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提交于 Jun 05 2012

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I am very pleased to hear you would like to accept our offer and test ab111574 in WB. This code will give you: 1 free PRIMARY ANTIBODY before the expiratio...

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I have confirmed that the antibody ab111574, raised against MCL1 pSer159, has not been tested for reactivity with MCL1 pSer159/pThr163.