Anti-MCL1抗体[RC13] (Biotin) (ab79473)

概述

  • 产品名称Anti-MCL1抗体[RC13] (Biotin)
    参阅全部 MCL1 一抗
  • 描述
    小鼠单克隆抗体[RC13] to MCL1 (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1 - 327 of Human MCL1

  • 阳性对照
    • Raji cell lysate. Spleen or tonsil tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab79473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
IHC-P
  • 应用说明IHC-P: 1/50, for 30 mins at RT. Perform heat mediated antigen retrieval before commencing with IHC staining protocol by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min, before immunostaining.
    IP: (denatured, with protein G) Use at 2 µg/mg of lysate.
    WB: Use at a concentration of 1 - 2 µg/ml for 2hrs at RT. Predicted molecular weight: 37 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
    • 序列相似性Belongs to the Bcl-2 family.
    • 翻译后修饰Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
      Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
      Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
    • 细胞定位Membrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Bcl 2 related protein EAT/mcl1 antibody
      • Bcl-2-like protein 3 antibody
      • Bcl-2-related protein EAT/mcl1 antibody
      • BCL2 related antibody
      • Bcl2-L-3 antibody
      • BCL2L3 antibody
      • EAT antibody
      • Induced myeloid leukemia cell differentiation protein Mcl 1 antibody
      • Induced myeloid leukemia cell differentiation protein Mcl-1 antibody
      • MCL 1 antibody
      • MCL1 antibody
      • MCL1-ES antibody
      • mcl1/EAT antibody
      • MCL1_HUMAN antibody
      • MCL1L antibody
      • MCL1S antibody
      • MGC104264 antibody
      • MGC1839 antibody
      • Myeloid Cell Leukemia 1 antibody
      • Myeloid cell leukemia ES antibody
      • Myeloid cell leukemia sequence 1 antibody
      • Myeloid cell leukemia sequence 1 BCL2 related antibody
      • Myeloid cell leukemia sequence 1 isoform 1 antibody
      • OTTHUMP00000032794 antibody
      • OTTHUMP00000032795 antibody
      • TM antibody
      see all

    Anti-MCL1 antibody [RC13] (Biotin) (ab79473)参考文献

    ab79473 has not yet been referenced specifically in any publications.

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