Anti-MCK10抗体(ab5508)

概述

  • 产品名称Anti-MCK10抗体
    参阅全部 MCK10 一抗
  • 描述
    兔多克隆抗体to MCK10
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids 31-47 of Human MCK10.

  • 阳性对照
    • Placenta lysate.
  • 常规说明


    Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Primary antibody说明Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 105 kDa).

靶标

  • 功能May be involved in cell-cell interactions and recognition.
  • 组织特异性Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 1 F5/8 type C domain.
    Contains 1 protein kinase domain.
  • 结构域The Gly/Pro-rich domains may be required for an unusual geometry of interaction with ligand or substrates.
  • 细胞定位Secreted and Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAK antibody
    • CD 167 antibody
    • CD167 antibody
    • CD167 antigen-like family member A antibody
    • CD167a antibody
    • Cell adhesion kinase antibody
    • DDR 1 antibody
    • DDR antibody
    • DDR1 antibody
    • DDR1_HUMAN antibody
    • Discoidin domain receptor antibody
    • Discoidin domain receptor tyrosine kinase 1 antibody
    • Discoidin receptor tyrosine kinase antibody
    • Discoidin receptor tyrosine kinase isoform a antibody
    • EDDR 1 antibody
    • EDDR1 antibody
    • Epithelial discoidin domain receptor 1 antibody
    • Epithelial discoidin domain-containing receptor 1 antibody
    • Epithelial specific receptor kinase antibody
    • HGK2 antibody
    • Mammarian carcinoma kinase 10 antibody
    • Mammary carcinoma kinase 10 antibody
    • MCK-10 antibody
    • MCK10 antibody
    • NEP antibody
    • Neuroepithelial tyrosine kinase antibody
    • Neurotrophic tyrosine kinase receptor type 4 antibody
    • NTRK 4 antibody
    • NTRK4 antibody
    • OTTHUMP00000029343 antibody
    • OTTHUMP00000029344 antibody
    • OTTHUMP00000029345 antibody
    • OTTHUMP00000029346 antibody
    • OTTHUMP00000029347 antibody
    • OTTHUMP00000164863 antibody
    • OTTHUMP00000164867 antibody
    • OTTHUMP00000222080 antibody
    • Protein-tyrosine kinase 3A antibody
    • Protein-tyrosine kinase RTK-6 antibody
    • PTK 3 antibody
    • PTK 3A protein tyrosine kinase 3A antibody
    • PTK3 antibody
    • PTK3A antibody
    • Receptor tyrosine kinase NEP antibody
    • RTK 6 antibody
    • RTK6 antibody
    • TRK E antibody
    • TRKE antibody
    • Tyrosine kinase DDR antibody
    • Tyrosine kinase receptor E antibody
    • Tyrosine-protein kinase CAK antibody
    see all

Anti-MCK10 antibody 图像



  • Predicted band size : 105 kDa


    ab5508 at a 1/100 dilution staining approximately 100kDa band of MCK 10 in placenta lysate by Western blot (ECL).
  • ab5508 staining MCK10 in human breast carcinoma (BC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-MCK10 antibody (ab5508)参考文献

ab5508 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam.

To our knowledge, ab5508 has not been tested in IP. If you have not purchased ab5508 yet, testing in IP would be eligible for our AbTrial program assuming you are testing in human samples. If you are not testing...

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That's super news that you find these two antibodies detect bands of the predicted size in transfected cells with the corresponding bands absent in your negative controls. Would you be ageeable to our posting the blot images on our online datahsheet?

We will send you a complementary vial of ab5508 and ab5520. There will be a short delay while we wait for these antibodies to be delivered from the originator. Your order will be despatched from our office in Cambridge, MA, USA. I look forward to r...

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We would be most interested in your testing these two antibodies as you describe. Please let me know the address to which they should be shipped.

As discussed in previous e-mail correspondence, we have changed the names of these two antibdies (ab5508 & ab5520) to anti DDR1 and DDR2 and have contacted the originator for further information. Like us, they are very grateful for your comments an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"