The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 - 18 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
MCHR, a member of the G protein coupled receptor family 1, is an integral plasma membrane protein which binds melanin concentrating hormone. MCHR can inhibit cAMP accumulation and stimulate intracellular calcium flux, and is probably involved in the neuronal regulation of food consumption. Although structurally similar to somatostatin receptors, MCHR does not seem to bind somatostatin.
ab115474 at 10-18ug/ml staining MCHR in Formalin-fixed, Paraffin-embedded Human brain (substantia nigra) tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen