Anti-mCherry抗体(ab205402)

概述

  • 产品名称
    Anti-mCherry抗体
    参阅全部 mCherry 一抗
  • 描述
    鸡多克隆抗体to mCherry
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 免疫原

    Recombinant full length protein (His-tag) corresponding to mCherry. The immunogen sequence is from a following reference. Shaner NC et al. Nature Biotechnology 22:1567-1572 (2004).

  • 阳性对照
    • Lysate of HEK293 cells transfected with pFin-EF1-mCherry vector; HEK293 cells transfected with pFin-EF1-mCherry vector.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • 纯度
    IgY fraction
  • 纯化说明
    Concentrated IgY preparation.
  • 克隆
    多克隆
  • 同种型
    IgY
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab205402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 30 kDa.
ICC/IF 1/1000.

靶标

  • 相关性
    mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.

图片

  • Immunofluorescent analysis of HEK293 cells transfected with pFin-EF1-mCherry vector labeling mCherry using ab205402 at 1/1000 dilution (green). Red: mCherry; Blue: blue DNA stain. Note: Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal.

  • Anti-mCherry antibody (ab205402) at 1/2000 dilution + lysate of HEK293 cells transfected with pFin-EF1-mCherry vector

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
  • Immunocytochemistry/ Immunofluorescence analysis of Human ES cells with mCherry gene reporter labeling mCherry with ab205402 at 1/1000 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X. Blocking was carried out with 10%BSA for 1 hour at 4°C, followed by incubation with ab205402 at 1/1000 for 12 hours at 4°C. A polyclonal goat anti-chicken Alexa Fluor® 488 was used as the secondary antibody at 1/100 diluton.

    See Abreview

文献

ab205402 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (H7.S6 Human ES cells with mCherry gene reporter)
Permeabilization
Yes - 0.1% Triton-X
Specification
H7.S6 Human ES cells with mCherry gene reporter
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde
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提交于 Aug 30 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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