概述

  • 产品名称
    Anti-mCherry抗体
    参阅全部 mCherry 一抗
  • 描述
    兔多克隆抗体to mCherry
  • 经测试应用
    适用于: ICC/IF, IHC - Wholemount, IP, WBmore details
  • 免疫原

    Recombinant fragment within mCherry aa 1-236. The exact sequence is proprietary.

  • 阳性对照
    • Recombinant mCherry, mCherry-tagged protein expressing 293T cells, mCherry fusion protein-transfected HeLa cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.0
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.5% BSA, 30% Glycerol, 69% PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab183628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC - Wholemount 1/100.
IP 1/3000.
WB 1/1000 - 1/10000. Predicted molecular weight: 27 kDa.

靶标

  • 相关性
    mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.

图片

  • Whole mount immunohistochemical analysis of 2 days-post-fertilization zebrafish embryos labelling mCherry-expressing cells with ab183628 at 1/100 dilution.

  • All lanes : Natural Human Cathepsin S protein (ab183268) at 1/2000 dilution

    Lane 1 : Full length recombinant mCherry at 0.005 µg
    Lane 2 : Full length recombinant mCherry at 0.01 µg
    Lane 3 : Full length recombinant mCherry at 0.02 µg


    Predicted band size : 27 kDa
  • All lanes : Natural Human Cathepsin S protein (ab183268) at 1/5000 dilution

    Lane 1 : 293T whole cell lysate/extract
    Lane 2 : Whole cell lysate/extract of mCherry-tagged protein expressing 293T cells

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 27 kDa
  • Immunoprecipitation analysis of 293T whole cell lysate/extract using ab183268 at a 1/3000 dilution.

    Lane 1: 20 μg whole cell lysate/extract of Cherry protein-expressing 293T cells.
    Lane 2: Control with 2.5 μg of pre-immune rabbit IgG.
    Lane 3: Immunoprecipitation of mCherry protein with 2.5 μg of ab183268.

    7.5% SDS-PAGE. An anti-rabbit IgG (HRP) was used as a secondary reagent.

  • Immunofluorescent analysis of mCherry fusion protein-transfected HeLa cells. Cells were fixed with Triton X-100 for 1 minute, then 4% paraformaldehyde for 30 minutes at room temperature. mCherry (green) was labelled using ab183628 at a dilution of 1/500.

    Red: mCherry fusion protein is expressed in the transfected cell.
    Blue: Hoechst 33342 staining.

  • Immunofluorescent analysis of mCherry fusion protein-transfected HeLa cells. Cells were fixed with Triton X-100 for 1 minute, then 4% paraformaldehyde for 30 minutes at room temperature. mCherry (green) was labelled using ab183628 at a dilution of 1/500.

    Red: mCherry fusion protein is expressed in the transfected cell.
    Blue: Hoechst 33342 staining.

文献

This product has been referenced in:
  • Heim JB  et al. Myosin-1E interacts with FAK proline-rich region 1 to induce fibronectin-type matrix. Proc Natl Acad Sci U S A 114:3933-3938 (2017). CHIPseq ; Mouse, Human . Read more (PubMed: 28348210) »
  • Huang A  et al. Decoding temporal interpretation of the morphogen Bicoid in the early Drosophila embryo. Elife 6:N/A (2017). IHC-P ; Drosophila melanogaster . Read more (PubMed: 28691901) »

See all 10 Publications for this product

客户评价及客户问答

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse Tumour cells expressing mCherry in the Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6 Citrate
Permeabilization
No
Specification
Mouse Tumour cells expressing mCherry in the Lung
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
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提交于 Mar 16 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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