概述

  • 产品名称
    Anti-mCherry抗体
    参阅全部 mCherry 一抗
  • 描述
    兔多克隆抗体to mCherry
  • 经测试应用
    适用于: WB, ICC/IF, IHC-Pmore details
  • 免疫原

    Recombinant full length protein corresponding to mCherry.

  • 阳性对照
    • HEK293 cells transfected with pFin- EF1-mCherry vector.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.06% Sodium azide
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab167453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000.
ICC/IF 1/500.
IHC-P Use a concentration of 3.33 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 相关性
    mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.

图片

  • Immunofluorescent analysis of HEK293 cells transfected with pFin- EF1-mCherry vector labeling mCherry with ab167453 at 1/500 (red). Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Blue DNA stain.
  • All lanes : Anti-mCherry antibody (ab167453) at 1/1000 dilution

    Lane 1 : HEK293 cells transfected with pFin- EF1-mCherry vector
    Lane 2 : Non-transfected HEK293 cells
  • Immunohistochemical analysis of paraffin-embedded mouse lung metastases sections labelling mCherry with ab167453 at a concentration of 3.33 µg/mL. The secondary antibody used was HRP conjugated goat anti rabbit at a concentration of 2 µg/mL. Antigen retrieval was heat mediated using Menapath access super pH 9.5.

    See Abreview

文献

This product has been referenced in:
  • Parenti N  et al. Direct imaging of APP proteolysis in living cells. PeerJ 5:e3086 (2017). WB, ICC/IF . Read more (PubMed: 28413720) »
  • Bressan RB  et al. Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural stem cells. Development 144:635-648 (2017). ICC/IF . Read more (PubMed: 28096221) »

See all 28 Publications for this product

客户评价及客户问答

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
Yes - 3% H2O2/Methanol for 10min, RT
Specification
Lung
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 15 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - 0.25% triton
Specification
U2OS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 24 2017

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Username

Abcam user community

Verified customer

提交于 Jan 24 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (H7.S6 Human ES cells with mCherry gene reporter)
Permeabilization
Yes - 0.1% Triton X
Specification
H7.S6 Human ES cells with mCherry gene reporter
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 30 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung metastases)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 9.5 Menapath access super
Permeabilization
No
Specification
Lung metastases
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Username

Mr. Bruce Fanshawe

Verified customer

提交于 Jun 03 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mCherry positive tumour cells in lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH: 6
Permeabilization
No
Specification
mCherry positive tumour cells in lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
10% Buffered Formalin
Username

Abcam user community

Verified customer

提交于 May 12 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Humen leukemia cells with mCherry in murine bone)
Permeabilization
Yes - 0.1% Tween
Specification
Humen leukemia cells with mCherry in murine bone
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Formalin
Username

Abcam user community

Verified customer

提交于 Apr 20 2015

Application
Western blot
Loading amount
0.1 µg
Gel Running Conditions
Reduced Denaturing (10 percent gel)
Sample
Human Purified protein (mCherry against various amounts of nanograms of pu)
Specification
mCherry against various amounts of nanograms of pu
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

提交于 Feb 11 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 48 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Sample
Mouse Cell (Brain tissue)
Specification
Brain tissue
Permeabilization
Yes - Triton 0.3%
Fixative
Formaldehyde
Username

Dr. Jenny Clarkson

Verified customer

提交于 Mar 04 2014

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step
None
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Karine Thibault

Verified customer

提交于 Feb 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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