Anti-mCherry抗体[1C51] (ab125096)

概述

  • 产品名称
    Anti-mCherry抗体[1C51]
    参阅全部 mCherry 一抗
  • 描述
    小鼠单克隆抗体[1C51] to mCherry
  • 经测试应用
    适用于: WB, ICC/IF, IHC-Fr, IHC-Pmore details
  • 免疫原

    Recombinant full length His tagged mCherry purified from E. coli.

  • 阳性对照
    • HEK293 crude extract transfected with pFin-EF1-mCherry; Hek293 cells transfected with mCherry.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.067% Sodium azide
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    1C51
  • 同种型
    IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab125096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Detects a band of approximately 28 kDa.
ICC/IF 1/500.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 相关性
    mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.

图片

  • Immunocytochemical analysis of HEK293 cells labelling mCherry which has been transfected into cells and labelled using ab125096 (with a red secondary). DAPI was used to stain DNA meaning only their nuclei are visualised in blue.

  • ab125096 staining mCherry in human pancreatic islet tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with Triton X-100 and blocked with BSA + Milk for 1 hour at 25°C. Samples were incubated with primary antibody (1/250) for 2 hours at 25°C. An Alexa Fluor® 488-conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab125096 staining mCherry in smooth muscle cells in rat bladder by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 10% buffered formalin and blocked with 5000 ug/ml BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat polyclonal anti mouse (1/400) was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Chen XW  et al. CYP4A in tumor-associated macrophages promotes pre-metastatic niche formation and metastasis. Oncogene N/A:N/A (2017). Mouse . Read more (PubMed: 28481877) »
  • San Gil R  et al. Using bicistronic constructs to evaluate the chaperone activities of heat shock proteins in cells. Sci Rep 7:2387 (2017). Read more (PubMed: 28539657) »

See all 35 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Kathryn Baker

Verified customer

提交于 Mar 15 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (xenograft of mCherry labelled human (HUVEC) cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9
Permeabilization
No
Specification
xenograft of mCherry labelled human (HUVEC) cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 12 2016

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Whole Head Lysate)
Specification
Whole Head Lysate
Blocking step
Odyssey as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 23 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain
Fixative
Paraformaldehyde
Username

Dr. Karine Thibault

Verified customer

提交于 Nov 04 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (BT474 Cells)
Specification
BT474 Cells
Permeabilization
Yes - 1% NP40
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 06 2014


I can confirm that we have tested this antibody with DsRed and it works well.

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA+Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Tissue sections (Pancreatic islet)
Specification
Pancreatic islet
Permeabilization
Yes - Triton X-100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 11 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Zebrafish Embryo (To detect exogenous mCherry expression in brain)
Specification
To detect exogenous mCherry expression in brain
Username

Abcam user community

Verified customer

提交于 Feb 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (mCherry labeled smooth muscle cells in Bladder)
Specification
mCherry labeled smooth muscle cells in Bladder
Fixative
10% Buffered Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: .01M Sodium Citrate
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5000µg/mL · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jan 29 2013

Thank you for your patience. The originator of the antibody anti-mCherry ab125096 has confirmed that it cross-reacts with mTomato. Please let us know if you have any other questions.

1-10 of 39 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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