Anti-MCG10抗体(ab63948)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to MCG10
  • 宿主
    Goat
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog
  • 免疫原

    Peptide with sequence C-NGSKKAERQKFSPY, from the C Terminus of the protein sequence according to NP_065151.1; NP_127501.1; NP_127502.1; NP_127503.1.

  • 阳性对照
    • HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab63948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 44 kDa).
ELISA 1/128000.

靶标

  • 相关性
    MCG10 is a member of the KH-domain RNA-binding protein family. Proteins of this family bind to RNA with specificity for C-rich pyrimidine regions. MCG10 is induced by p53 and DNA damage via two p53-responsive promoter elements.
  • 数据库链接
  • 别名
    • Alpha CP4 antibody
    • LIP4 antibody
    • LYST interacting protein antibody
    • PCBP4 antibody
    • poly(rC) binding protein 4 antibody
    • rCbinding protein 4 antibody
    • RNA binding protein MCG10 antibody
    see all

图片

  • Anti-MCG10 antibody (ab63948) at 1 µg/ml (1 hour) + HeLa cell lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 41 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab63948 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
CLIP
Sample
Human Cell lysate - whole cell (HEK 293T)
Specification
HEK 293T
Type
iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control
hnRNP C antibody (sc-15386) bound protein G dynabeads 0.1 mg/ml (antibody/beads).
Negative control
Protein G dynabeads without antibody bound.
Immuno-precipitation step
Other - Protein G dynabeads
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提交于 Aug 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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