MCF7全细胞裂解液(ab3871)

概述

  • 产品名称MCF7全细胞裂解液
    参阅全部 MCF7 细胞裂解液
  • 常规说明Source - MCF7 cells (human) from ATCC (cat# HTB-22).


    MCF7 whole cell lysate was prepared by homogenization in modified RIPA buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 1 mM EDTA, 1 mM PMSF,5 µg/ml Aprotinin, 1 µg/ml Pepstatin-A, 2 µg/ml Leupeptin, 1 mM Na3VO4, 1 mM NaF). Debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • 经测试应用适用于: WBmore details

性能

  • 存放说明Store at -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Constituents: 5% Bromophenol blue, 2% Beta mercaptoethanol, 4.4% SDS, 7.9% Tris HCl, 20% Glycerol
  • Concentration information loading...
  • 裂解物说明MCF7 whole cell lysate was prepared by homogenization in modified RIPA buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 1 mM EDTA, 1 mM PMSF,5 µg/ml Aprotinin, 1 µg/ml Pepstatin-A, 2 µg/ml Leupeptin, 1 mM Na3VO4, 1 mM NaF). Debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • 研究领域
  • 背景MCF-7 cells are a well-characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin , endothelin , GFAP, neurofilament, vimentin.

应用

Our Abpromise guarantee covers the use of ab3871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. MCF7 whole cell lysate is ready to load on SDS-PAGE for Western blotting. We recommend loading 20 µg per lane for mini gels.

MCF7 whole cell lysate (ab3871)参考文献

This product has been referenced in:
  • Lau JY  et al. Differential expression of peroxisome proliferator activated receptor gamma and cyclin D1 does not affect proliferation of asthma- and non-asthma-derived airway smooth muscle cells. Respirology 15:303-12 (2010). WB . Read more (PubMed: 20070588) »

See 1 Publication for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for contacting us.

I am sorry to hear that you are having some trouble with the antibody. I am sending you a free positive control lysate of MCF7 cells, ab3871. To check the status of this order, please call Customer Service at 888-...

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Thank you for contacting us.

As we discussed on the phone, you may be able to improve your WB results by switching from milk to BSA and by using a higher concentration of primary antibody (1:500 for example). If you are unable to improve your...

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Thank you for contacting Abcam regarding ab58803.

I am sorry that you have been experiencing difficulties with this antibody in WB. As you discussed with my colleague on the phone, I look forward to hearing back from you after you have teste...

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Thank you for the clarification. That is an unusual result, if the DDR1 in the MCF7 is intact. We have not received other complaints about the lysate. We do not have a different lot of ab3871, so I suggest trying a similar product, ab2953...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"