MCF7核extract裂解液(H2O2 treated) (ab14862)

概述

  • 产品名称MCF7核extract裂解液(H2O2 treated)
    参阅全部 MCF7 细胞裂解液
  • 描述
    MCF7核extract裂解液
  • 常规说明The MCF-7 nuclear extracts have been collected in Lysis Buffer after 3 hrs incubation with or without 0.2 mM hydrogen peroxide. The protein content has been determined by a Bradford-based assay.

性能

  • 形式Liquid
  • 存放说明Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 400% Sodium chloride, 20% Glycerol, Protease inhibitor, 20mM HEPES, 10mM Sodium fluoride, 10mM Beta glycerophosphate, 1mM Sodium metavanadate, 1mM Para nitrophenyl phosphate, 1mM DTT, 0.1mM EDTA, 0.01mM Sodium molybdate. pH 7.5
  • Concentration information loading...
  • 研究领域
  • 背景MCF-7 cells are a well-characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin , endothelin , GFAP, neurofilament, vimentin.

MCF7 nuclear extract lysate (H2O2 treated) 图像

  •  Each lot has been tested for p53 activation by using TransAM™ p53 Kit. The signal intensity for p53 activation in each lot is compared to the signal intensity obtained with extracts from untreated cells. Once the signals are blanked, the ratio between the signals from extracts of treated cells over untreated cells used at 5 µg/well is above 4. This ratio may vary depending on the basal level of p53 activation in a given cell type.

     

MCF7 nuclear extract lysate (H2O2 treated) (ab14862)参考文献

ab14862 has not yet been referenced specifically in any publications.

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