MCF7核extract裂解液(H2O2 post treated) (ab14863)

概述

  • 产品名称MCF7核extract裂解液(H2O2 post treated)
    参阅全部 MCF7 细胞裂解液
  • 描述
    MCF7核extract裂解液
  • 常规说明Post-collection treatment consists in exposing the nuclear extract to a final concentration of 200 mM H2O2 for 20 min on ice. The protein content has been determined by a Bradford-based assay.

性能

  • 形式Liquid
  • 存放说明Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 20% Glycerol, Protease inhibitor, 400mM Sodium chloride, 20mM HEPES, 10mM Sodium fluoride, 10mM Para nitrophenyl phosphate, 10mM Beta glycerophosphate, 1mM DTT, 1mM Sodium metavanadate, 0.1mM EDTA, 0.01mM Sodium molybdate. pH 7.5
  • Concentration information loading...
  • 研究领域
  • 背景MCF-7 cells are a well-characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin , endothelin , GFAP, neurofilament, vimentin.

MCF7 nuclear extract lysate (H2O2 post treated) 图像

  • Each lot has been tested for Sp1 activity by using the TransAM™ Sp1 Kit. The signal intensity for Sp1 activity in each lot is compared to the signal intensity obtained with H2O2-post treated extracts from MCF-7 cells. Once the signals are blanked, the ratio between the signals from untreated extracts over treated extracts used at 5 µg/well must be above 4.

MCF7 nuclear extract lysate (H2O2 post treated) (ab14863)参考文献

ab14863 has not yet been referenced specifically in any publications.

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We do not know what effect H2O2 stress will have on expression of BCL9 in MCF7 cells therefore I would recommend perhaps running H2O2 treated MCF7 lysate alongside untreated if possible to determine affect. Read More

Thank you for your enquiry. MCF stands for Michigan Cancer Foundation, the Detroit institution that derived this cell line. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. The MCF-7 nuclear extracts have been collected in Lysis Buffer. The Lysis Buffer consists of 20 mM Hepes pH 7.5, 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 µM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM ß-glyceropho...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"