MCF7膜extract裂解物(ab29539)
概述
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产品名称
MCF7膜extract裂解物
参阅全部 MCF7 细胞裂解液 -
常规说明
Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting. -
存储溶液
Constituents: 0.1% Bromophenol blue, 0.1% Magnesium chloride, 1.17% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.1% HEPES, 0.1% EDTA, 10% Glycerol, 0.1% Sodium chloride -
Concentration information loading...
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裂解物说明
Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. -
研究领域
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背景
MCF-7 cells are a well-characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin , endothelin , GFAP, neurofilament, vimentin.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab29539于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.
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说明 |
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WB
Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab29539 尚未被引用在任何文献中。