概述

  • 产品名称Anti-MCAK (phospho S95)抗体
    参阅全部 MCAK 一抗
  • 描述
    兔多克隆抗体to MCAK (phospho S95)
  • 特异性Antibody detects endogenous levels of MCAK when only phosphorylated at serine 95.
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human MCAK around the phosphorylation site of serine 95 (R-R-SP-V-N).

  • 阳性对照
    • Extract from HeLa cells (treated with TNF at 10ng/ml for 30mins). A549 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS(without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab74146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能Promotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.
  • 组织特异性Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific.
  • 序列相似性Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
    Contains 1 kinesin-motor domain.
  • 发展阶段Isoform 2 is expressed in fetal testis.
  • 结构域The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.
  • 细胞定位Cytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4930402F02Rik antibody
    • CT139 antibody
    • ESTM5 antibody
    • KIF 2C antibody
    • kif2c antibody
    • KIF2C_HUMAN antibody
    • Kinesein Family Member 2C antibody
    • Kinesin family member 2C antibody
    • kinesin like 6 (mitotic centromere associated kinesin) antibody
    • Kinesin like 6 antibody
    • Kinesin like protein 6 antibody
    • Kinesin like protein KIF2C antibody
    • Kinesin-like protein 6 antibody
    • Kinesin-like protein KIF2C antibody
    • KNS L6 antibody
    • KNSL 6 antibody
    • KNSL6 antibody
    • MCAK antibody
    • MGC11883 antibody
    • Mitotic centromere associated kinesin antibody
    • Mitotic centromere-associated kinesin antibody
    • OTTHUMP00000010066 antibody
    • X83316 antibody
    see all

Anti-MCAK (phospho S95) antibody 图像

  • ab74146, at 1/500 dilution, staining A549 cells using immunofluorescence analysis, in the absence and presence of the immunising phosphopeptide.
  • All lanes : Anti-MCAK (phospho S95) antibody (ab74146) at 1/500 dilution

    Lane 1 : HeLa cell extract, treated with TNF (10ng/ml, 30mins),
    Lane 2 : HeLa cell extract, treated with TNF (10ng/ml, 30mins), with immunising phosphopeptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 81 kDa
    Observed band size : 81 kDa

Anti-MCAK (phospho S95) antibody (ab74146)参考文献

This product has been referenced in:
  • Lin BW  et al. Overexpression of Aurora-C interferes with the spindle checkpoint by promoting the degradation of Aurora-B. Cell Death Dis 5:e1106 (2014). WB ; Human . Read more (PubMed: 24603334) »
  • Pakala SB  et al. Signaling-dependent phosphorylation of mitotic centromere-associated kinesin regulates microtubule depolymerization and its centrosomal localization. J Biol Chem 287:40560-9 (2012). ICC/IF ; Human . Read more (PubMed: 23055517) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (MEF)
Gel Running Conditions Reduced Denaturing (7.5%)
Loading amount 50 µg
Treatment Nocodazole 16 hrs
Specification MEF
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 18 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"