概述

  • 产品名称
  • 描述
    兔多克隆抗体to MCAK
  • 经测试应用
    适用于: IHC-P, IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Chimpanzee, Ferret, Rhesus monkey, Chinese hamster
  • 免疫原

    Synthetic peptide from a region between residue 50 and 100 of Human MCAK using the numbering given in entry NP_006836.1

  • 阳性对照
    • Whole lysate from HeLa cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab70536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 应用说明
    Is unsuitable for WB.
  • 靶标

    • 功能
      Promotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.
    • 组织特异性
      Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific.
    • 序列相似性
      Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
      Contains 1 kinesin-motor domain.
    • 发展阶段
      Isoform 2 is expressed in fetal testis.
    • 结构域
      The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
    • 翻译后修饰
      Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.
    • 细胞定位
      Cytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 4930402F02Rik antibody
      • CT139 antibody
      • ESTM5 antibody
      • KIF 2C antibody
      • kif2c antibody
      • KIF2C_HUMAN antibody
      • Kinesein Family Member 2C antibody
      • Kinesin family member 2C antibody
      • kinesin like 6 (mitotic centromere associated kinesin) antibody
      • Kinesin like 6 antibody
      • Kinesin like protein 6 antibody
      • Kinesin like protein KIF2C antibody
      • Kinesin-like protein 6 antibody
      • Kinesin-like protein KIF2C antibody
      • KNS L6 antibody
      • KNSL 6 antibody
      • KNSL6 antibody
      • MCAK antibody
      • MGC11883 antibody
      • Mitotic centromere associated kinesin antibody
      • Mitotic centromere-associated kinesin antibody
      • OTTHUMP00000010066 antibody
      • X83316 antibody
      see all

    Anti-MCAK antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma (left) and breast carcinoma (right) tissues labelling MCAK with ab70536 at 1/1000 (1µg/ml). Detection: DAB.

    Anti-MCAK antibody (ab70536)参考文献

    ab70536 has not yet been referenced specifically in any publications.

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