Anti-MC4 Receptor抗体(ab24233)

概述

  • 产品名称Anti-MC4 Receptor抗体
    参阅全部 MC4 Receptor 一抗
  • 描述
    兔多克隆抗体to MC4 Receptor
  • 经测试应用适用于: WB, IHC-P, ELISA, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    YGLHSNASESLGK

    , corresponding to amino acids 21-33 of Mouse MC4 Receptor

  • 常规说明


    This antibody mainly detects an unglycosylated protein at 37kDa and a glycosylated protein at 55kDa.

性能

应用

Our Abpromise guarantee covers the use of ab24233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).Can be blocked with Mouse MC4 Receptor peptide (ab78212). A larger band is also detected at 55 kDa. This most likely represents the glycosylated form of the protein.
IHC-P Use a concentration of 1 µg/ml.
ELISA Use a concentration of 0.4 µg/ml.
IHC-Fr 1/1000.

靶标

  • 功能Receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. This receptor is mediated by G proteins that stimulate adenylate cyclase.
  • 组织特异性Brain, placental, and gut tissues.
  • 疾病相关Defects in MC4R are a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MC4-R antibody
    • MC4R antibody
    • MC4R_HUMAN antibody
    • Melanocortin 4 receptor antibody
    • Melanocortin receptor 4 antibody
    • MGC126851 antibody
    • MGC138197 antibody
    see all

Anti-MC4 Receptor antibody 图像

  • ab24233 staining MC4 Receptor in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 21°C. Samples were incubated with primary antibody (1/1000 in TPBS + normal goat serum) for 19 hours at 4°C. A biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-MC4 Receptor antibody (ab24233)

    Lane 1 : Human cerebella cortex pellet.
    Lane 2 : Human hippocampus homogenate.

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 37 kDa
    Additional bands at : 55 kDa (possible glycosylated form).
  • Immunoperoxidase staining of rat brain section with ab24233. Note the tadpole like staining of cortex neurons.

Anti-MC4 Receptor antibody (ab24233)参考文献

This product has been referenced in:
  • Peter JC  et al. A pharmacologically active monoclonal antibody against the human melanocortin-4 receptor: effectiveness after peripheral and central administration. J Pharmacol Exp Ther 333:478-90 (2010). WB, IP ; Human . Read more (PubMed: 20118207) »
  • Chitravanshi VC  et al. Microinjections of alpha-melanocyte stimulating hormone into the nucleus ambiguus of the rat elicit vagally mediated bradycardia. Am J Physiol Regul Integr Comp Physiol 296:R1402-11 (2009). IHC-FoFr ; Rat . Read more (PubMed: 19297540) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Sample Rat Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
Username

Mr. Thomas Groves

Verified customer

提交于 Jul 23 2013

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Mouse Cell (Kupffer Cells)
Specification Kupffer Cells
Permeabilization Yes - 0.3% Triton X
Fixative Acetone
Username

Abcam user community

Verified customer

提交于 Oct 24 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Rat Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.3% Triton X
Fixative Acetone
Username

Abcam user community

Verified customer

提交于 Oct 24 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Mouse Tissue lysate - whole (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Oct 24 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (HepG2)
Specification HepG2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Oct 24 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Rat Tissue lysate - whole (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Oct 24 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Rat Cell (hepatocytes)
Specification hepatocytes
Permeabilization Yes - 0.3% Triton X
Fixative Acetone
Username

Abcam user community

Verified customer

提交于 Oct 24 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Mouse Tissue sections (liver)
Specification liver
Permeabilization Yes - 0.3% Triton X
Fixative Acetone
Username

Abcam user community

Verified customer

提交于 Oct 24 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Rat Cell (kupffer cells)
Specification kupffer cells
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 28 2013

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Human Cell (HepG2)
Specification HepG2
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 28 2013

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