重组
RabMAb

Anti-MC1 Receptor抗体[EPR6530] (ab125031)

概述

  • 产品名称
    Anti-MC1 Receptor抗体[EPR6530]
    参阅全部 MC1 Receptor 一抗
  • 描述
    兔单克隆抗体[EPR6530] to MC1 Receptor
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human MC1 Receptor.

  • 阳性对照
    • WB: A375 and Human melanoma lysates IHC-P: Human melanoma tissue
  • 常规说明

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab125031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
IHC-P 1/500 - 1/1000. Antigen retrieval is not essential but may optimise staining.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 功能
      Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
    • 组织特异性
      Melanocytes and corticoadrenal tissue.
    • 疾病相关
      Genetic variations in MC1R are a cause of susceptibility to cutaneous malignant melanoma type 5 (CMM5) [MIM:613099]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
    • 序列相似性
      Belongs to the G-protein coupled receptor 1 family.
    • 细胞定位
      Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CMM5 antibody
      • MC1-R antibody
      • MC1R antibody
      • Melanocortin 1 receptor antibody
      • Melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor) antibody
      • Melanocortin receptor 1 antibody
      • Melanocyte-stimulating hormone receptor antibody
      • Melanotropin receptor antibody
      • MSH-R antibody
      • MSHR antibody
      • MSHR_HUMAN antibody
      • SHEP2 antibody
      see all

    图片

    • All lanes : Anti-MC1 Receptor antibody [EPR6530] (ab125031) at 1/1000 dilution

      Lane 1 : A375 cell lysate
      Lane 2 : Human melanoma lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size: 35 kDa

    • ab125031, at a 1/500 dilution, staining MC1 Receptor in paraffin embedded Human melanoma tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    文献

    This product has been referenced in:
    • Chen F  et al. Melanocortin-1 Receptor-Targeting Ultrasmall Silica Nanoparticles for Dual-Modality Human Melanoma Imaging. ACS Appl Mater Interfaces 10:4379-4393 (2018). Read more (PubMed: 29058865) »

    See 1 Publication for this product

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Specification
    skin
    Fixative
    Formaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 12 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (skin biopsy and cells)
    Loading amount
    20 µg
    Specification
    skin biopsy and cells
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (5-16% gel)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 12 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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