Anti-MBD4抗体(ab13793)

概述

  • 产品名称Anti-MBD4抗体
    参阅全部 MBD4 一抗
  • 描述
    兔多克隆抗体to MBD4
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    This antibody was generated by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 268-282 (

    CNKADAESEPVAQKS

    ) and 337-352 (CSAKDSEHNEKYEDTF) of human MBD4.

  • 阳性对照
    • HL-60 cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明This antibody is affinity purified.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab13793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 70 kDa).

靶标

  • 功能Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.
  • 序列相似性Contains 1 MBD (methyl-CpG-binding) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3 N(4) ethenocytosine glycosylase antibody
    • G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody
    • G/T mismatch glycosylase antibody
    • G/U mismatch glycosylase antibody
    • MBD 4 antibody
    • MBD4 antibody
    • MBD4_HUMAN antibody
    • MED 1 antibody
    • MED1 antibody
    • Methyl CpG binding domain protein 4 antibody
    • Methyl CpG binding endonuclease 1 antibody
    • Methyl CpG binding protein MBD4 antibody
    • Methyl-CpG-binding domain protein 4 antibody
    • Methyl-CpG-binding endonuclease 1 antibody
    • Methyl-CpG-binding protein MBD4 antibody
    • Mismatch specific DNA N glycosylase antibody
    • Mismatch-specific DNA N-glycosylase antibody
    • Putative methyl CpG binding protein antibody
    see all

Anti-MBD4 antibody 图像



  • Predicted band size : 70 kDa


    ab13793, at 2 ug/ml, staining approximately 64 kDa human MBD4 in HL-60 cell lysate by Western blot.

Anti-MBD4 antibody (ab13793)参考文献

ab13793 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for answering my questions. I'm sorry to hear you are having a problem with ab13793. I would like to suggest the following modifications to your protocol: Please try blocking with only 5% milk in TBST. 10% seems high to me, ...

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Thank you for your enquiry. Could you please tell me how you blocked the membrane? Also, did you add blocking agent to your primary antibody? I hope this information helps, please do not hesitate to contact us if you need any more advice or info...

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