Anti-MBD3抗体- Carboxyterminal end (ab71777)

概述

  • 产品名称Anti-MBD3抗体- Carboxyterminal end
    参阅全部 MBD3 一抗
  • 描述
    兔多克隆抗体to MBD3 - Carboxyterminal end
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the C-terminal region of human MBD3.

  • 阳性对照
    • Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue and transfected/ non-transfected 293 cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Ammonium Sulphate Precipitation
  • 纯化说明This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab71777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

靶标

  • 功能Does not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing.
  • 序列相似性Contains 1 MBD (methyl-CpG-binding) domain.
  • 细胞定位Nucleus. Nuclear, in discrete foci.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI181826 antibody
    • AU019209 antibody
    • MBD 3 antibody
    • Mbd3 antibody
    • MBD3: methyl CpG binding domain protein 3 antibody
    • MBD3_HUMAN antibody
    • Methyl CpG binding domain protein 3 antibody
    • Methyl CpG binding protein MBD3 antibody
    • Methyl-CpG-binding domain protein 3 antibody
    • Methyl-CpG-binding protein MBD3 antibody
    see all

Anti-MBD3 antibody - Carboxyterminal end 图像

  • All lanes : Anti-MBD3 antibody - Carboxyterminal end (ab71777) at 1/100 dilution

    Lane 1 : Non-transfected 293 cell lysates
    Lane 2 : MBD3 transfected 293 cell lysates

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 33 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab71777 at a 1:50 dilution. A peroxidase-conjugated secondary antibody was then used, followed by DAB staining.

Anti-MBD3 antibody - Carboxyterminal end (ab71777)参考文献

ab71777 has not yet been referenced specifically in any publications.

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