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Recombinant full length protein (Drosophilia melanogaster).
Methyl CpG binding domain proteins (MBDs) are a family of mammalian proteins that specifically bind methylated regions of DNA. There are two methyl CpG binding complexes, MeCP1 and MeCP2. MeCP2 is comprised of a single protein also denoted MeCP2. MeCP1 is composed of four different proteins termed MBD 1 (also known as PCM 1), MBD 2, MBD 3, and MBD 4 (also known as MED 1).
Our Abpromise guarantee covers the use of ab3757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500. Detects a band of approximately 25,35 kDa (predicted molecular weight: 25,35 kDa).
Detects two bands of approximately 25 kDa and 35 kDa which correspond to the predicted molecular weights of recombinant drosophila MBD 2 + 3 short and long forms, respectively.
Rabbit polyclonal to Drosophila MBD 2 + 3 (at a 1/500 dilution):
1. Recombinant drosophila MBD 2 + 3 (long form)
2. Recombinant drosophila MBD 2 + 3d (short form)
3. Nuclear extract of Drosophila embryos where the two drosophila MBD 2 + 3 forms are detected.
ab3757 has not yet been referenced specifically in any publications.
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