The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/500.
Epitope exposure with citrate buffer will enhance staining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
Highly expressed in brain, heart, kidney, stomach, testis and placenta.
Contains 1 MBD (methyl-CpG-binding) domain.
Nucleus. Nuclear, in discrete foci. Detected at replication foci in late S phase.
ab84753 at 1/100 dilution staining MBD2 in human breast carcinoma by Immunohistochemistry/Immunofluorescence, Formalin-fixed, Paraffin-embedded tissue. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1:100.