概述

  • 产品名称Anti-MBD2抗体
    参阅全部 MBD2 一抗
  • 描述
    兔多克隆抗体to MBD2
  • 特异性This antibody is specific for MBD2 (N term). Rat - Abreview feedback has stated that this antibody works on rat prostate. It gives some non-specific band in rat testis, probably due to presence of other MBD isoforms.
  • 经测试应用适用于: ICC/IF, IP, ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A KLH conjugated synthetic peptide (10-30 aa in length) in the region of 160-177 of MBD2 (Human)

  • 阳性对照
    • A375 cell lysate and mouse brain tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab38646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/50.
IP Use at an assay dependent concentration.
ELISA 1/1000.
WB 1/100 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
  • 组织特异性Highly expressed in brain, heart, kidney, stomach, testis and placenta.
  • 序列相似性Contains 1 MBD (methyl-CpG-binding) domain.
  • 细胞定位Nucleus. Nuclear, in discrete foci. Detected at replication foci in late S phase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Demethylase antibody
    • DMTase antibody
    • MBD 2 antibody
    • Mbd2 antibody
    • MBD2_HUMAN antibody
    • MBD2a antibody
    • Methyl CpG binding domain protein 2 antibody
    • Methyl CpG binding protein MBD2 antibody
    • Methyl-CpG-binding domain protein 2 antibody
    • Methyl-CpG-binding protein MBD2 antibody
    • NY CO 41 antibody
    see all

Anti-MBD2 antibody 图像

  • ab38646 immunoprecipitating MBD2 in human K562 whole cell lysate. 15µg of cell lysate was incubated with primary antibody (2 µg/ml in RIPA buffer) and matrix (Protein A/G) for 12 hours at 4°C. For western blotting a HRP-conjugated goat anti-rabbit IgG polyclonal (1/2500) was used as the secondary antibody.

    See Abreview

  • ab38646 staining MBD2 in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 10 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/500 in TBS) for 12 hours at 4°C. An undiluted alkaline phosphatase-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-MBD2 antibody (ab38646) at 1/1000 dilution

    Lane 1 : A375 cell lysate
    Lane 2 : mouse brain tissue lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • ab38646 staining MBD2 in rat liver cultured cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 1% BSA for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/50 for 10 hours at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 568 (red) used at a 1/400 dilution.

Anti-MBD2 antibody (ab38646)参考文献

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Mouse Cell (MEL)
Specification MEL
Permeabilization No
Fixative Paraformaldehyde
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Abcam user community

Verified customer

提交于 May 13 2014

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Human Cell lysate - whole cell (K562)
Specification K562
Total protein in input 15 µg
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Abcam user community

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提交于 Apr 16 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
Sample Human Tissue sections (normal tonsil)
Specification normal tonsil
Permeabilization No
Fixative Paraformaldehyde
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Abcam user community

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提交于 Nov 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Sample Mouse Cell lysate - whole cell (MEL, 293T cell)
Specification MEL, 293T cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Dr. Min young Kim

Verified customer

提交于 Jun 25 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Rat Cell lysate - nuclear (Rat Neuronal Cortex)
Specification Rat Neuronal Cortex
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step Semiquantitative PCR
Positive control Mecp2 antibody.
Negative control purified rabbit IgG
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提交于 Nov 27 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Liver, cultured cell line)
Specification Liver, cultured cell line
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Abcam user community

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提交于 Feb 06 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Rat Cell lysate - whole cell (Liver)
Specification Liver
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde (1% final concent
Detection step Real-time PCR
Positive control anti-acetyl Histone H3
Negative control normal rabbit IgG
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Abcam user community

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提交于 Nov 12 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Prostate (ventrale lobe))
Loading amount 5 µg
Specification Prostate (ventrale lobe)
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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提交于 Jun 22 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"