概述

描述

  • 性质Synthetic

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技术指标

Our Abpromise guarantee covers the use of ab4918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Blocking

  • 形式Liquid
  • 补充说明


    This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and human MBD 1, catalog ab2846. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~ 70-fold) for competitive inhibition of antibody-protein binding reactions.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

常规信息

  • 别名
    • CXXC 3
    • CXXC-type zinc finger protein 3
    • CXXC3
    • MBD 1
    • Mbd1
    • MBD1_HUMAN
    • MECP1 COMPLEX
    • Methyl CpG binding domain protein 1
    • Methyl CpG binding domain protein 1 isoform PCM1
    • Methyl CpG binding protein MBD1
    • Methyl CpG binding protein splice variant 1
    • Methyl CpG binding protein splice variant 2
    • Methyl CpG binding protein splice variant 3
    • Methyl CpG binding protein splice variant 4
    • Methyl-CpG-binding domain protein 1
    • Methyl-CpG-binding protein MBD1
    • PCM 1
    • PCM1
    • Protein containing methyl-CpG-binding domain 1
    • Regulator of fibroblast growth factor 2 (FGF 2) transcription
    • RFT
    • The regulator of fibroblast growth factor 2(FGF 2) transcription
    see all
  • 功能Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
  • 组织特异性Widely expressed.
  • 序列相似性Contains 3 CXXC-type zinc fingers.
    Contains 1 MBD (methyl-CpG-binding) domain.
  • 结构域The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
    The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.
    The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.
  • 翻译后修饰Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.
  • Information by UniProt

MBD1 peptide (ab4918)参考文献

This product has been referenced in:

See 1 Publication for this product

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The binding with methylated DNA is related to the peptide ab4918.Please chek this is not a prtoein it is a blocking peptide whihc was used to producty antibody ab2846. This is not a fucntional peptide.

The...

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The peptide is dissolved in water without any salts. For storage in your lab, just aliquot.

Thank you for your inquiry.

MBD1 :

I would like to point out that ab4918 is 13 amino acid synthetic peptide whose sequences are derived from human MBD1 protein, C-R(593)SKDLKKPGARK(605) (amino to carboxyl terminus). This peptide can...

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Thanks for sending the link. Unfortunately we don't have a subscription so I was not able to open the paper. Do you have a PDF version of the paper?

Thank you for your enquiry. ab4918 is not a functional peptide of any kind. It is just a short peptide used as the immunogen to produce Anti-MBD1 antibody - ChIP Grade (ab2846). So it will not bind 5-methyl cytosine on DNA. I hope this ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"