概述

  • 产品名称Anti-MBD1抗体[EPR3564]
    参阅全部 MBD1 一抗
  • 描述
    兔单克隆抗体[EPR3564] to MBD1
  • 经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)

  • 阳性对照
    • HeLa, A431, 293T and Jurkat cell lysates; Human tonsil tissue; MCF7 cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab108510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa).
IP 1/20.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
    • 组织特异性Widely expressed.
    • 序列相似性Contains 3 CXXC-type zinc fingers.
      Contains 1 MBD (methyl-CpG-binding) domain.
    • 结构域The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
      The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.
      The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.
    • 翻译后修饰Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CXXC 3 antibody
      • CXXC-type zinc finger protein 3 antibody
      • CXXC3 antibody
      • MBD 1 antibody
      • Mbd1 antibody
      • MBD1_HUMAN antibody
      • MECP1 COMPLEX antibody
      • Methyl CpG binding domain protein 1 antibody
      • Methyl CpG binding domain protein 1 isoform PCM1 antibody
      • Methyl CpG binding protein MBD1 antibody
      • Methyl CpG binding protein splice variant 1 antibody
      • Methyl CpG binding protein splice variant 2 antibody
      • Methyl CpG binding protein splice variant 3 antibody
      • Methyl CpG binding protein splice variant 4 antibody
      • Methyl-CpG-binding domain protein 1 antibody
      • Methyl-CpG-binding protein MBD1 antibody
      • PCM 1 antibody
      • PCM1 antibody
      • Protein containing methyl-CpG-binding domain 1 antibody
      • Regulator of fibroblast growth factor 2 (FGF 2) transcription antibody
      • RFT antibody
      • The regulator of fibroblast growth factor 2(FGF 2) transcription antibody
      see all

    Anti-MBD1 antibody [EPR3564] 图像

    • All lanes : Anti-MBD1 antibody [EPR3564] (ab108510) at 1/500 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 67 kDa
    • ab108510, at 1/100 dilution, staining MBD1 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
    • ab108510, at 1/100 dilution, staining MBD1 in MCF7 cells by Immunofluorescence.

    Anti-MBD1 antibody [EPR3564] (ab108510)参考文献

    This product has been referenced in:
    • Hata K  et al. DNA methylation and methyl-binding proteins control differential gene expression in distinct cortical areas of macaque monkey. J Neurosci 33:19704-14 (2013). Macaque Monkey . Read more (PubMed: 24336734) »

    See 1 Publication for this product

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